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Information on EC 2.4.3.4 - beta-galactoside alpha-2,3-sialyltransferase
for references in articles please use BRENDA:EC2.4.3.4
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.3 Sialyltransferases
2.4.3.4 beta-galactoside alpha-2,3-sialyltransferase
IUBMB Comments
The acceptor is Galβ1,3GalNAc-R, where R is H, a threonine or serine residue in a glycoprotein, or a glycolipid. Lactose can also act as acceptor. May be identical with EC 2.4.3.2 β-D-galactosyl-(1→3)-N-acetyl-β-D-galactosaminide α-2,3-sialyltransferase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
showSynonyms
st3gal, alpha-2,3-sialyltransferase, alpha 2,3-st, beta-galactoside alpha-2,3-sialyltransferase, st3gala.1, beta-galactoside alpha-2,3-sialyltransferase 1, siatfl, galbeta1,3galnac alpha2,3-sialyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,3SiaT
alpha 2,3-ST
-
-
-
-
alpha2-3-sialyltransferase 1
-
multifunctional enzyme which has alpha2-6-sialyltransferase, alpha2-3-sialidase, and alpha2-3-trans-sialidase activities in addition to its major alpha2-3-sialyltransferase activity
alpha2->3 sialyltransferase
-
-
-
-
alpha3-SA-T
-
-
-
-
alpha3SA-T
beta-D-Gal-(1-3)-D-GalNAc alpha-(2-3)-sialyltransferase
-
-
-
-
beta-galactoside alpha-2,3-sialyltransferase
-
-
-
-
CMP-N-acetylneuraminate:beta-D-galactoside alpha-2,3-N-acetylneuraminyl-transferase
-
-
-
-
CMP-N-acetylneuraminate:beta-D-galactoside alpha2-3 sialyltransferase
-
-
-
-
CMP-Neu5Ac:Glbeta1-3GalNAc alpha2,3-sialyltransferase
-
-
-
-
CMP-NeuAc:galactoside(alpha2-3)-sialyltransferase
-
-
-
-
CMP-SA:Galbeta3GalNAc-R alpha3-sialyltransferase
-
-
-
-
CMP-sialic acid:Galbeta1-3GalNAc-R alpha3-sialyltransferase
-
-
-
-
EC 2.4.99.4
-
-
formerly
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Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
-
-
-
-
Gal-NAc6S
-
-
-
-
Galbeta1,3GalNAc alpha2,3-sialyltransferase
galbeta1,3galNAcalpha2,3-sialyltransferase
-
-
-
-
NeuAc alpha-2,3-sialyltransferase
-
-
-
-
sialyltransferase, cytidine monophosphoacetylneuraminate-beta-galactoside alpha2-->3-
-
-
-
-
SIATFL
-
-
-
-
ST3Gal I
ST3Gal III
ST3GalA.1
-
-
-
-
ST3GalIA
-
-
-
-
ST3O
-
-
-
-
alpha3SA-T
-
-
-
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Galbeta1,3GalNAc alpha2,3-sialyltransferase
-
-
-
-
ST3Gal I
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
ganglio-series glycosphingolipids biosynthesis, globo-series glycosphingolipids biosynthesis, mucin core 1 and core 2 O-glycosylation
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:beta-D-galactoside alpha-(2->3)-N-acetylneuraminyl-transferase
The acceptor is Galbeta1,3GalNAc-R, where R is H, a threonine or serine residue in a glycoprotein, or a glycolipid. Lactose can also act as acceptor. May be identical with EC 2.4.3.2 beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3-sialyltransferase.
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CAS REGISTRY NUMBER
COMMENTARY
71124-51-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP + 3-O-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc]-L-tyrosyl-[protein]
CMP-N-acetyl-beta-neuraminate + 3-O-[beta-D-Gal-(1->3)-alpha-D-GalNAc]-L-tyrosyl-[protein]
Substrates: the enzyme synthesizes CMP-NeuAc by transferring NeuAc from the alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-unit of O-glycans
Products: -
Products: -
r
CMP-N-(4-pentynoyl)neuraminic acid + asialofetuin
?
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + 2-nitrophenyl D-galactose
?
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a type 3 histo-blood group antigen precursor disaccharide
an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R + CMP + H+
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
CMP + ?
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + benzyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + benzyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)(GlcNAcbeta(1->6))GalNAc-OBn
CMP + ?
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)GalNAc-OBn
CMP + Neu5Acalpha(2->3)Galbeta(1->3)GalNAc-OBn
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + 3'-sialyllactose + N-acetyl-beta-D-neuraminate
-
Substrates: -
Products: -
Products: -
r
CMP-N-acetyl-beta-neuraminate + octyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + octyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [porcine submaxillary asialo-mucin]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [porcine submaxillary asialo-mucin]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [porcine submaxillary asialo-mucin]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [porcine submaxillary asialo-mucin]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
CMP + 4-methylumbelliferyl alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucoside
-
Substrates: best substrate, about 37% conversion
Products: -
Products: -
?
CMP-N-acetylneuraminate + 4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
CMP + 4-nitrophenyl alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetylneuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc
CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Substrates: best acceptor substrate
Products: -
Products: -
?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
Substrates: the enzyme is involved in O-linked glycosylation pathway for synthesis of core 1 and core 2 oligosaccharides
Products: -
Products: -
?
CMP-N-acetylneuraminate + lactose
CMP + NeuAcalpha(2->3)Galbeta(1->4)Glc
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + asialofetuin
CMP + ?
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + asialofetuin
CMP + ?
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + alpha2,3-SiaLac + Neu5Ac
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + alpha2,3-SiaLac + Neu5Ac
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
additional information
?
-
Substrates: MeO-3-Galbeta(1->3)GalNAc-OBn is a poor acceptor for the enzyme
Products: -
Products: -
-
additional information
?
-
-
Substrates: MeO-3-Galbeta(1->3)GalNAc-OBn is a poor acceptor for the enzyme
Products: -
Products: -
-
additional information
?
-
-
Substrates: no activity with fetuin or alpha-1-acid glycoprotein (asialoorosomucoid)
Products: -
Products: -
-
additional information
?
-
Substrates: with glycoprotein and ganglioside acceptors the substrate specificity is strict, with the sequence beta-D-Gal-(1->3)-alpha-D-GalNAc serving as the exclusive acceptor. The enzyme has an equilibrium random order mechanism
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a type 3 histo-blood group antigen precursor disaccharide
an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R + CMP + H+
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + lactose
CMP + 3'-sialyllactose + N-acetyl-beta-D-neuraminate
-
Substrates: -
Products: -
Products: -
r
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [antifreeze glycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-serine
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + [asialoglycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
CMP + [asialoglycoprotein]-(O-3-(alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
Substrates: the enzyme specifically modifies glycoproteins and glycolipids which contain the sequence beta-D-Gal-(1->3)-beta-D-GalNAc to form the sequence alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc
Products: -
Products: -
?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
Substrates: the enzyme is involved in O-linked glycosylation pathway for synthesis of core 1 and core 2 oligosaccharides
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
-
Substrates: -
Products: -
Products: -
?
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
Substrates: -
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Sodium citrate
direct sialylation using CMP-N-acetyl-beta-neuraminate
additional information
-
overexpression of N-acetylglucosaminyltransferase III significantly inhibits alpha2,3-sialylation
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
lysophosphatidylcholine
C16-18 acyl derivatives of lysophosphatidylcholine stimulate the activity of enzyme form A more extensively than the C14 acyl derivative, and the C12 acyl derivative is without effect on enzyme form A
phospholipid/octylglucoside liposomes
incorporation into phospholipid/octylglucoside liposomes activates enzyme form A
-
Triton X-100
stimulates activity of enzyme form A. Enzyme form B is fully active in absence of detergents. Detergents stimulate the activity of enzyme form A by lowering the KD and KM of CMP-N-acetylneuraminate and increasing the Vmax of the reaction
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.02 - 1.388
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
0.04903
CMP-N-(4-pentynoyl)neuraminic acid
-
recombinant enzyme DELTA56ST3GalI, at pH 6.2 and 37°C
0.008
Galbeta(1->3)(GlcNAcbeta(1->6))GalNAc-OBn
isoform ST3Gal-I, at pH 6.0 and 25°C
0.35 - 0.39
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
-
0.54
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.67
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.71
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
1.4
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.02
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
0.026
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
0.096
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
0.105
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
0.211
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
0.352
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
0.444
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
0.549
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
0.854
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
1.388
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
0.04123
CMP-N-acetyl-beta-neuraminate
-
recombinant enzyme DELTA56ST3GalI, at pH 6.2 and 37°C
0.121
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
0.176
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
0.18
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
0.184
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
0.205
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
0.271
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.481
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
0.668
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
0.876
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
0.18
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.34
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.44
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.62
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.35
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form A
-
0.39
[antifreeze glycoprotein]-(O-3-(beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->)))-L-threonine
pH 6.5, 37°C, enzyme form B
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.2 - 3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
2 - 8
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
17
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
19
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
47
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.2
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
0.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
0.65
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
0.8
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
2.28
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
2.32
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
2.42
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
2.97
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
3.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
0.13
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.62
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
0.63
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
1.05
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
1.21
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
2.25
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
2.5
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
2.7
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
3.65
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
19
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
23
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
26
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
32
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.37 - 121.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
28
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
32
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
34
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
39
4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
0.37
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315Y, at pH 7.4 and 37°C
1.05
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y191A, at pH 7.4 and 37°C
1.75
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme V315A, at pH 7.4 and 37°C
3.47
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230A, at pH 7.4 and 37°C
3.78
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N147S, at pH 7.4 and 37°C
6.77
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme N170A, at pH 7.4 and 37°C
9.85
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme Y230F, at pH 7.4 and 37°C
22.07
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme S148A, at pH 7.4 and 37°C
114.17
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
121.15
benzyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside
wild type enzyme, at pH 7.4 and 37°C
0.47
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315Y, at pH 7.4 and 37°C
0.72
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y191A, at pH 7.4 and 37°C
1.87
CMP-N-acetyl-beta-neuraminate
mutant enzyme N147S, at pH 7.4 and 37°C
3.35
CMP-N-acetyl-beta-neuraminate
mutant enzyme N170A, at pH 7.4 and 37°C
5.83
CMP-N-acetyl-beta-neuraminate
mutant enzyme V315A, at pH 7.4 and 37°C
7.58
CMP-N-acetyl-beta-neuraminate
mutant enzyme S148A, at pH 7.4 and 37°C
12.2
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230A, at pH 7.4 and 37°C
15.33
CMP-N-acetyl-beta-neuraminate
mutant enzyme Y230F, at pH 7.4 and 37°C
18.6
CMP-N-acetyl-beta-neuraminate
mutant enzyme C59S/C64S, at pH 7.4 and 37°C
30
CMP-N-acetylneuraminate
-
mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
69
CMP-N-acetylneuraminate
-
mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
73
CMP-N-acetylneuraminate
-
wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
140
CMP-N-acetylneuraminate
-
mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
T-lymphocytes, Epstein-Barr virus immortalized B-lymphocytes
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM