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Information on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase and Organism(s) Mycobacterium tuberculosis and UniProt Accession O06401

for references in articles please use BRENDA:EC2.4.2.28
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.28 S-methyl-5'-thioadenosine phosphorylase
IUBMB Comments
Also acts on 5'-deoxyadenosine and other analogues having 5'-deoxy groups.
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Select one or more organisms in this record: ?
This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: O06401
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Word Map
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
methylthioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, mta phosphorylase, mtap protein, 5'-deoxy-5'-methylthioadenosine phosphorylase, ssmtapii, ssmtap, mtapase, mesado phosphorylase, pfmtap, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5'-deoxy-5'-methylthioadenosine phosphorylase
-
-
-
-
5'-methylthioadenosine nucleosidase
-
-
-
-
5'-methylthioadenosine phosphorylase
-
-
-
-
MeSAdo phosphorylase
-
-
-
-
MeSAdo/Ado phosphorylase
-
-
-
-
methylthioadenosine nucleoside phosphorylase
-
-
-
-
methylthioadenosine phosphorylase
-
-
-
-
MTA phosphorylase
-
-
-
-
MTAPase
-
-
-
-
phosphorylase, methylthioadenosine
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5'-thioadenosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase
Also acts on 5'-deoxyadenosine and other analogues having 5'-deoxy groups.
CAS REGISTRY NUMBER
COMMENTARY hide
61970-06-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-methylthioadenosine + phosphate
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
adenosine + phosphate
adenine + D-ribose 1-phosphate
show the reaction diagram
2.1% activity compared to activity with 5'-methylthioadenosine
-
-
?
S-adenosyl-L-homocysteine + phosphate
?
show the reaction diagram
0.81% activity compared to activity with 5'-methylthioadenosine
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-methylthio-DADMe-immucillin A
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
5'-methylthioadenosine
pH and temperature not specified in the publication, co-substrate: 50 mM phosphate
0.26
phosphate
pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
5'-methylthioadenosine
pH and temperature not specified in the publication, co-substrate: 50 mM phosphate
0.26
phosphate
pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000025 - 0.000075
5'-methylthio-DADMe-immucillin A
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
pH 6.5: about 55% of maximal activity, pH 9.0: about 55% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 30000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
4h, 90% loss of activity
4
4h, 40% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Buckoreelall, K.; Sun, Y.; Hobrath, J.V.; Wilson, L.; Parker, W.B.
Identification of Rv0535 as methylthioadenosine phosphorylase from Mycobacterium tuberculosis
Tuberculosis
92
139-147
2012
Mycobacterium tuberculosis (O06401), Mycobacterium tuberculosis
Manually annotated by BRENDA team