Information on EC 2.4.2.15 - guanosine phosphorylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.2.15
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RECOMMENDED NAME
GeneOntology No.
guanosine phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
guanosine + phosphate = guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
guanine and guanosine salvage
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guanosine nucleotides degradation III
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nucleoside and nucleotide degradation (archaea)
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purine ribonucleosides degradation
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purine metabolism
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Purine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
guanosine:phosphate alpha-D-ribosyltransferase
Also acts on deoxyguanosine.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-28-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
deoxyguanosine + phosphate
guanine + deoxyribose 1-phosphate
show the reaction diagram
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-
-
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guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
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enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
PCMB
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0.2 mM, complete inhibition
Tris buffer
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0.1 M, pH 7.5, 22% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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required for optimal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
deoxyguanosine
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0.216
guanosine
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0.376
phosphate
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reaction with guanosine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.4
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phosphorolysis of guanosine
7
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phosphorolysis of deoxyguanosine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
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pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10 C, stable for 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (4041 entries)
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