Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required for optimal activity | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 0.2 mM, complete inhibition | Oryctolagus cuniculus | |
Tris buffer | 0.1 M, pH 7.5, 22% inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
deoxyguanosine | - |
Oryctolagus cuniculus | |
0.216 | - |
guanosine | - |
Oryctolagus cuniculus | |
0.376 | - |
phosphate | reaction with guanosine | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine + phosphate | Oryctolagus cuniculus | enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood | guanine + D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Oryctolagus cuniculus | - |
intestinal mucosa | - |
Oryctolagus cuniculus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.87 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-10 C, stable for 1 month | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + deoxyribose 1-phosphate | - |
? | |
guanosine + phosphate | equilibrium constant is 0.019 | Oryctolagus cuniculus | guanine + D-ribose 1-phosphate | - |
r | |
guanosine + phosphate | enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood | Oryctolagus cuniculus | guanine + D-ribose 1-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
phosphorolysis of deoxyguanosine | Oryctolagus cuniculus |
7 | 7.4 | phosphorolysis of guanosine | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum | Oryctolagus cuniculus |