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Information on EC 2.4.1.68 - glycoprotein 6-alpha-L-fucosyltransferase and Organism(s) Sus scrofa and UniProt Accession P79282

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase
IUBMB Comments
This enzyme catalyses a reaction similar to that of EC 2.4.1.214, glycoprotein 3-alpha-L-fucosyltransferase, but transfers the L-fucosyl group from GDP-beta-L-fucose to form an alpha1,6-linkage rather than an alpha1,3-linkage.
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This record set is specific for:
Sus scrofa
UNIPROT: P79282
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Word Map
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  • 2.4.1.68
  • fucosylation
  • n-glycans
  • glcnac
  • antibody-dependent
  • alpha1,6-fucosylation
  • innermost
  • biantennary
  • fcgammariiia
  • alpha1,3-fucosyltransferase
  • non-fucosylated
  • emphysema-like
  • core-fucosylated
  • medicine
  • alpha1,2
  • synthesis
The taxonomic range for the selected organisms is: Sus scrofa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
alpha1,6-fucosyltransferase, fucosyltransferase 8, alpha1-6fuct, alpha-1,6-fucosyltransferase, alpha1,6fuct, 6fuct, alpha(1,6)ft, alpha6fuct, alpha(1,6)fucosyltransferase, alpha1,6-fuct, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha1-6FucT
-
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fucosyltransferase, guanosine diphosphofucose-glycoprotein
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-
-
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GDP-fucose glycoprotein fucosyltransferase
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-
-
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GDP-fucose-glycoprotein fucosyltransferase
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-
-
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GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1-6fucosyltransferase
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-
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GDP-L-fucose-glycoprotein fucosyltransferase
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-
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GDPfucose glycoprotein fucosyltransferase
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-
-
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glycoprotein fucosyltransferase
-
-
-
-
guanosine diphosphofucose-glycoprotein fucosyltransferase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = GDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc]-L-asparaginyl-[protein]
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase
This enzyme catalyses a reaction similar to that of EC 2.4.1.214, glycoprotein 3-alpha-L-fucosyltransferase, but transfers the L-fucosyl group from GDP-beta-L-fucose to form an alpha1,6-linkage rather than an alpha1,3-linkage.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-08-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminy]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
-
i.e. GnGn-peptide
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminy]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
-
i.e. GnGn-peptide
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?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mn2+
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slight inhibition
N-ethylmaleimide
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not affected by
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.046
GDP-L-fucose
0.0174 - 0.025
N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000039
-
recombinant enzyme from COS-1 cells
4.37
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
liver microsomes
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FUT8_PIG
575
1
66229
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
4 - 9
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-
489336
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrile
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10%, stable
Methanol
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10%, stable
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C to 37°C: 24 h, no loss of activity
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
large scale, from brain by affinity chromatography with GDP-hexanolamine resin and GnGn-bi-Asn-resin
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partially from several organs
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in COS-1 cells, DNA sequence determination and analysis
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Uozumi, N.; Teshima, T.; Yamamoto, T.; Nishikawa, A.; Gao, Y.E.; Miyoshi, E.; Gao, C.X.; Noda, K.; Islam, K.N.; et al.
A fluorescent assay method for GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-6fucosyltransferase activity, involving high performance liquid chromatography
J. Biochem.
120
385-392
1996
Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Uozumi, N.; Yanagidani, S.; Miyoshi, E.; Ihara, Y.; Sakuma, T.; Gao, C.X.; Teshima, T.; Fujii, S.; Shiba, T.; Taniguchi, N.
Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1->6fucosyltransferase
J. Biol. Chem.
271
27810-27817
1996
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Yanagidani, S.; Uozumi, N.; Ihara, Y.; Miyoshi, E.; Yamaguchi, N.; Taniguchi, N.
Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells
J. Biochem.
121
626-632
1997
Homo sapiens, Homo sapiens (Q9BYC5), Sus scrofa
Manually annotated by BRENDA team
Struppe, E.; Staudacher, E.
Occurrence of GDP-L-fucose:beta-N-acetylglucosamine (Fuc to Asn-linked GlcNAc) alpha1,6-fucosyltransferases in porcine, sheep, bovine, rabbit and chicken tissues
Biochim. Biophys. Acta
1475
360-368
2000
Bos taurus, Gallus gallus, Oryctolagus cuniculus, Ovis aries, Sus scrofa
Manually annotated by BRENDA team
Breton, C.; Oriol, R.; Imberty, A.
Conserved structural features in eukaryotic and prokaryotic fucosyltransferases
Glycobiology
8
87-94
1998
Azorhizobium caulinodans (Q43966), Bradyrhizobium japonicum (Q45271), Homo sapiens (Q9BYC5), Sus scrofa (P79282)
Manually annotated by BRENDA team
Miyoshi, E.; Noda, K.; Yamaguchi, Y.; Inoue, S.; Ikeda, Y.; Wang, W.; Ko, J.H.; Uozumi, N.; Li, W.; Taniguchi, N.
The alpha1-6-fucosyltransferase gene and its biological significance
Biochim. Biophys. Acta
1473
9-20
1999
Homo sapiens, Homo sapiens (Q9BYC5), Rattus norvegicus, Sus scrofa, Sus scrofa (P79282)
Manually annotated by BRENDA team