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Information on EC 2.4.1.211 - 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase and Organism(s) Cutibacterium acnes and UniProt Accession B7XEJ1
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2.4.1.211 1,3-beta-galactosyl-N-acetylhexosamine phosphorylaseIUBMB Comments
Reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product.
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Word Map
- 2.4.1.211
-
glutamine-binding
- longum
-
ligand-free
- l-glutamine
-
4-epimerase
- synthesis
-
uridylyltransferase
- galnac
-
bifidobacterial
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ligand-bound
- bifidus
- phosphorylases
- biotechnology
- nutrition
- medicine
The taxonomic range for the selected organisms is: Cutibacterium acnes
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
glnbp, lacto-n-biose phosphorylase, galacto-n-biose/lacto-n-biose i phosphorylase, gl-bp, gnb/lnb phosphorylase, beta-1,3-galactosyl-n-acetylhexosamine phosphorylase, galacto-n-biose phosphorylase, lacto-n-biose i phosphorylase, galhexnacp, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-D-galactopyranosyl-(1-3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase
Reaction also occurs with beta-D-galactopyranosyl-(1->3)-N-acetyl-D-galactosamine as the substrate, giving N-acetyl-D-galactosamine as the product.
CAS REGISTRY NUMBER
COMMENTARY
224427-06-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine
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r
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
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-
r
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
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-
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?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
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-
-
?
additional information
?
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GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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-
?
additional information
?
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GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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?
additional information
?
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GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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-
?
additional information
?
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GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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-
?
additional information
?
-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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-
?
additional information
?
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-
GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
140
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
1300
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
11
N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
130
N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
103
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
14
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
42
N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
5.9
N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.73
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
0.01
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
3.9
N-acetyl-D-galactosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
0.044
N-acetyl-D-glucosamine
in 100 mM MOPS buffer (pH 7.0) at 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
83480
His-tagged enzyme, calculated from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakajima, M.; Nishimoto, M.; Kitaoka, M.
Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes
Appl. Microbiol. Biotechnol.
83
109-115
2009
Cutibacterium acnes (B7XEJ0), Cutibacterium acnes (B7XEJ1), Cutibacterium acnes, Cutibacterium acnes JCM 6425 (B7XEJ1), Cutibacterium acnes JCM 6473 (B7XEJ0)
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