Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cutibacterium acnes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
130 | - |
N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
140 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
1300 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
His-tagged enzyme, SDS-PAGE | Cutibacterium acnes |
83480 | - |
His-tagged enzyme, calculated from amino acid sequence | Cutibacterium acnes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cutibacterium acnes | B7XEJ0 | strain JCM6473 | - |
Cutibacterium acnes | B7XEJ1 | strain JCM6425 | - |
Cutibacterium acnes JCM 6425 | B7XEJ1 | strain JCM6425 | - |
Cutibacterium acnes JCM 6473 | B7XEJ0 | strain JCM6473 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Cutibacterium acnes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | - |
Cutibacterium acnes | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | Cutibacterium acnes | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine | r | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | Cutibacterium acnes JCM 6425 | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | specific activity on N-acetyl-D-galactosamine is approximately 60times higher than that on N-acetyl-D-glucosamine | r | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | - |
Cutibacterium acnes JCM 6473 | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Cutibacterium acnes | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | Cutibacterium acnes | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
r | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | GnpA shows approximately 70times higher specific activity of phosphorolysis on beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine than that on beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | Cutibacterium acnes JCM 6425 | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
r | |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | - |
Cutibacterium acnes JCM 6473 | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
additional information | GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate | Cutibacterium acnes | ? | - |
? | |
additional information | GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate | Cutibacterium acnes JCM 6425 | ? | - |
? | |
additional information | GnpA shows a weak activity on alpha-N-acetylgalactosaminyl-O-serine and does not exhibit significant activity (kcat less than 0.1 s-1) on D-galactose, D-glucose, glucosamine, 2-deoxy-glucose, D-fructose, mannose, maltose, and sucrose, N-acetylgalactosamine 6-sulfate, and N-acetylglucosamine 6-sulfate, GnpA does not act on either D-glucose 1-phosphate or N-acetyl-D-glucosamine 1-phosphate | Cutibacterium acnes JCM 6473 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-galactosyl-N-acetylhexosamine phosphorylase | - |
Cutibacterium acnes |
galacto-N-biose phosphorylase | - |
Cutibacterium acnes |
GalHexNAcP | - |
Cutibacterium acnes |
GnpA | - |
Cutibacterium acnes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cutibacterium acnes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme is stable up to 37°C | Cutibacterium acnes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
14 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
42 | - |
N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
103 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cutibacterium acnes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Cutibacterium acnes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
0.044 | - |
N-acetyl-D-glucosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
0.73 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes | |
3.9 | - |
N-acetyl-D-galactosamine | in 100 mM MOPS buffer (pH 7.0) at 37°C | Cutibacterium acnes |