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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Hordeum vulgare and UniProt Accession P31922

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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This record set is specific for:
Hordeum vulgare
UNIPROT: P31922
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Word Map
The taxonomic range for the selected organisms is: Hordeum vulgare
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, mtsucs1, susy1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
-
-
-
-
sucrose synthetase
-
-
-
-
sucrose-UDP glucosyltransferase
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-
-
-
sucrose-uridine diphosphate glucosyltransferase
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-
-
-
Sus
-
-
-
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UDP-glucose-fructose glucosyltransferase
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-
-
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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-
-
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uridine diphosphoglucose-fructose glucosyltransferase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
ADP-alpha-D-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
CDP + sucrose
CDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
CDP-alpha-D-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
dTDP + sucrose
dTDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dTDP-alpha-D-glucose + D-fructose
dTDP + sucrose
show the reaction diagram
-
-
-
-
r
dUDP + sucrose
dUDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dUDP-alpha-D-glucose + D-fructose
dUDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP + sucrose
GDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
GDP-alpha-D-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
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-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
the reaction is catalyzed at high pH values
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-
r
UDP + sucrose
UDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants
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-
r
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
the reaction is catalyzed at high pH values
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
ADP
30 - 210
sucrose
0.22
UDP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
285
ADP
-
at pH 7.0 and 37°C
285 - 435
sucrose
435
UDP
-
at pH 7.0 and 37°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform SS1
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
endosperm
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
inner side of cell membrane
-
Manually annotated by BRENDA team
within the cytoplasm and along cytoplasmic tracks
Manually annotated by BRENDA team
associated with mitochondrion
Manually annotated by BRENDA team
inner side of cell membrane
-
Manually annotated by BRENDA team
within the cytoplasm and along cytoplasmic tracks
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SUS1_HORVU
807
0
92211
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
92200
x * 92200, calculated
92300
x * 92300, calculated
98700
x * 98700, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 92200, calculated
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoform SS1 is upregulated by anoxia and by cold teperatures
expression of isoform SS1 is upregulated by anoxia and by cold temperatures
expression of isoform SS3 is upregulated by water deprivation and by cold teperatures
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Baroja-Fernandez, E.; Munoz, F.J.; Saikusa, T.; Rodriguez-Lopez, M.; Akazawa, T.; Pozueta-Romero, J.
Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants
Plant Cell Physiol.
44
500-509
2003
Hordeum vulgare, Solanum tuberosum
Manually annotated by BRENDA team
Barrero-Sicilia, C.; Hernando-Amado, S.; Gonzalez-Melendi, P.; Carbonero, P.
Structure, expression profile and subcellular localisation of four different sucrose synthase genes from barley
Planta
234
391-403
2011
Hordeum vulgare (C6H0M2), Hordeum vulgare (F8SM21), Hordeum vulgare (P31922), Hordeum vulgare
Manually annotated by BRENDA team
Schmoelzer, K.; Gutmann, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Sucrose synthase A unique glycosyltransferase for biocatalytic glycosylation process development
Biotechnol. Adv.
34
88-111
2016
Anabaena sp., Arabidopsis thaliana, Beta vulgaris, Vicia faba, Helianthus tuberosus, Hordeum vulgare, Ipomoea batatas, Manihot esculenta, Nitrosomonas europaea, Oryza sativa, Vigna radiata, Pisum sativum, Prunus persica, Pyrus pyrifolia, Saccharum sp., Solanum tuberosum, Zea mays, Thermosynechococcus vestitus, Acidithiobacillus caldus, Solanum chmielewskii, Glycine max (P13708)
Manually annotated by BRENDA team