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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Melioribacter roseus and UniProt Accession I7A3T6

for references in articles please use BRENDA:EC2.4.1.13
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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This record set is specific for:
Melioribacter roseus
UNIPROT: I7A3T6
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The taxonomic range for the selected organisms is: Melioribacter roseus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, mtsucs1, susy1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
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sucrose synthetase
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sucrose-UDP glucosyltransferase
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sucrose-uridine diphosphate glucosyltransferase
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Sus
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SuSy
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UDP-glucose-fructose glucosyltransferase
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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uridine diphosphoglucose-fructose glucosyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
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r
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
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r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
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r
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NDP-glucose + D-fructose
NDP + sucrose
show the reaction diagram
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r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
over 40% of maximal enzyme activity within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
purified recombinant His-tagged enzyme, without substrates, 15 min, 38% activity remaining
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene SuSyMr, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Diricks, M.; De Bruyn, F.; Van Daele, P.; Walmagh, M.; Desmet, T.
Identification of sucrose synthase in nonphotosynthetic bacteria and characterization of the recombinant enzymes
Appl. Microbiol. Biotechnol.
99
8465-8474
2015
Acidithiobacillus caldus (A0A059ZV61), Acidithiobacillus caldus, Denitrovibrio acetiphilus (D4H6M0), Denitrovibrio acetiphilus, Melioribacter roseus (I7A3T6), Melioribacter roseus, Nitrosomonas europaea (Q820M5), Nitrosomonas europaea, Nitrosomonas europaea ATCC 19718 (Q820M5), Denitrovibrio acetiphilus DSM 12809 (D4H6M0), Melioribacter roseus JCM 17771 (I7A3T6), Acidithiobacillus caldus ATCC 51756 (A0A059ZV61)
Manually annotated by BRENDA team