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Information on EC 2.3.2.15 - glutathione gamma-glutamylcysteinyltransferase and Organism(s) Caenorhabditis elegans and UniProt Accession A5JYS0

for references in articles please use BRENDA:EC2.3.2.15
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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.15 glutathione gamma-glutamylcysteinyltransferase
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This record set is specific for:
Caenorhabditis elegans
UNIPROT: A5JYS0 not found.
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Word Map
The taxonomic range for the selected organisms is: Caenorhabditis elegans
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nspcs, cepcs, phytochelatin synthase, atpcs1, ospcs1, ospcs2, atpcs2, atpcs, phytochelatin synthase 1, ljpcs1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
gamma-glutamylcysteine dipeptidyl transpeptidase
-
-
-
-
phytochelatin synthase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
gamma-glutamylcysteinyl transfer
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
glutathione:poly(4-glutamyl-cysteinyl)glycine 4-glutamylcysteinyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
125390-02-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + [Glu(-Cys)]n-Gly
Gly + [Glu(-Cys)]n+1-Gly
show the reaction diagram
G5ECE4
critical for heavy metal tolerance
-
-
?
glutathione + [Glu(-Cys)]n-Gly
Gly + [Glu(-Cys)]n+1-Gly
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutathione + [Glu(-Cys)]n-Gly
Gly + [Glu(-Cys)]n+1-Gly
show the reaction diagram
G5ECE4
critical for heavy metal tolerance
-
-
?
glutathione + [Glu(-Cys)]n-Gly
Gly + [Glu(-Cys)]n+1-Gly
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
activity assay
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCS1_CAEEL
426
0
48236
Swiss-Prot
Mitochondrion (Reliability: 4)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transformed into Saccharomyces cerevisiae strain DTY67, hypersensitive to Cd2+-stress
G5ECE4
expressed in Schizosaccharomyces pombe strain SP27, a phytochelatin synthase knockout strain
-
into the binary plasmid vector pCB302 for introducing into Agrobacterium GV3101 strain, tobacco plants are transformed by the standard leaf disc method
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Clemens, S.; Schroeder, J.I.; Degenkolb, T.
Caenorhabditis elegans expresses a functional phytochelatin synthase
Eur. J. Biochem.
268
3640-3643
2001
Caenorhabditis elegans
Manually annotated by BRENDA team
Vatamaniuk, O.K.; Bucher, E.A.; Ward, J.T.; Rea, P.A.
A new pathway for heavy metal detoxification in animals. Phytochelatin synthase is required for cadmium tolerance in Caenorhabditis elegans
J. Biol. Chem.
276
20817-20820
2001
Caenorhabditis elegans (G5ECE4), Caenorhabditis elegans, Caenorhabditis elegans N2 (G5ECE4)
Manually annotated by BRENDA team
Wojas, S.; Clemens, S.; Hennig, J.; Sklodowska, A.; Kopera, E.; Schat, H.; Bal, W.; Antosiewicz, D.M.
Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium
J. Exp. Bot.
59
2205-2219
2008
Arabidopsis thaliana, Caenorhabditis elegans
Manually annotated by BRENDA team