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Information on EC 2.3.1.77 - triacylglycerol-sterol O-acyltransferase for references in articles please use BRENDA:EC2.3.1.77
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EC Tree
IUBMB Comments Tripalmitoylglycerol and, more slowly, other triacylglycerols containing C6 to C22 fatty acids, can act as donors. The best acceptors are 3beta-hydroxysteroids with a planar ring system.
The expected taxonomic range for this enzyme is: Sinapis alba
Synonyms
acyltransferase, triacylglycerol-sterol, triacylglycerol:sterol acyltransferase,
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acyltransferase, triacylglycerol-sterol
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triacylglycerol:sterol acyltransferase
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triacylglycerol + a 3beta-hydroxysteroid = diacylglycerol + a 3beta-hydroxysteroid ester
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Acyl group transfer
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triacylglycerol:3beta-hydroxysterol O-acyltransferase
Tripalmitoylglycerol and, more slowly, other triacylglycerols containing C6 to C22 fatty acids, can act as donors. The best acceptors are 3beta-hydroxysteroids with a planar ring system.
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triacylglycerol + cholest-5-en-3beta-ol
diacylglycerols + cholesteryl ester
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triacylglycerol from olive oil
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trilinoleoylglycerol + cholest-5-en-3beta-ol
dilinoleoylglycerol + cholesteryl linoleate
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trioleoylglycerol + cholest-5-en-3beta-ol
dioleoylglycerol + cholesteryl oleate
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tripalmitoylglycerol + 24R-methylcholest-5-en-3beta-ol
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i.e. campestero l
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tripalmitoylglycerol + 5alpha-androstan-3beta-ol
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i.e. androstanol
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tripalmitoylglycerol + 5alpha-cholestan-3beta-ol
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i.e. cholestanol, highest rate of esterification
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tripalmitoylglycerol + 5beta-cholestan-3beta-ol
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i.e. coprostanol
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tripalmitoylglycerol + cholest-5-en-3beta-ol
dipalmitoylglycerol + cholesteryl palmitate
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i.e.cholesterol
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tripalmitoylglycerol + stigmast-5-en-3beta-ol
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i.e. sitosterol
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tripalmitoylglycerol + stigmasta-5,22-dien-3beta-ol
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i.e. stigmasterol
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tristearoylglycerol + cholest-5-en-3beta-ol
distearoylglycerol + cholesteryl stearate
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additional information
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additional information
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enzyme has an absolute requirement for a beta-configuration of the hydroxyl group at C-3, rate of esterification is higher for sterols containing a planar ring system, triacylglycerols with unsaturated fatty acids are used more efficiently than fatty acids saturated with equal chain length, triacylglycerols with fatty acids C6-C22 can be used as acyl donors
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additional information
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the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain
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additional information
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the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain
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additional information
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divalent metal ions have no stimulatory effect
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additional information
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reagents containing free sulfhydryl groups e.g. DTT have no effect
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additional information
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specific activity in seedlings after various days of germination
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white mustard
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brenda
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brenda
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of seedling
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germinating
brenda
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brenda
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additional information
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two peaks, one more than 100000 Da, the other 18000 Da: the fraction with higher molecular weight may consist of the aggregated form of the enzyme of 18000 Da
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purification has to be carried out at 0-4°C
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4°C, solubilized enzyme preparation, 24 h, 10% loss of activity
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Kalinowska, M.; Wojciechowski, Z.A.
Sterol conjugate interconversions during germination of white mustard (Sinapis alba)
Phytochemistry
23
2485-2488
1984
Sinapis alba
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brenda
Zimowski, J.; Wojciechowski, Z.A.
Partial purification and specificity of triacylglycerol: sterol acyltransferase from Sinapsis alba
Phytochemistry
20
1799-1803
1981
Sinapis alba
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brenda
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