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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
acetyltransferase, D-amino acid, D-amino acid acetyltransferase, D-amino acid-alpha-N-acetyltransferase, DNT, Hpa3p,
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acetyltransferase, D-amino acid
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D-amino acid acetyltransferase
D-amino acid-alpha-N-acetyltransferase
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D-amino acid acetyltransferase
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D-amino acid acetyltransferase
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acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid
acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid
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acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid
ordered bi bi mechanism with acetyl-CoA being the first substrate to be bound and CoA the last product to be released, involving acetylation of Cys304
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Acyl group transfer
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acetyl-CoA:D-amino-acid N-acetyltransferase
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acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
acetyl-CoA + D-alpha-aminobutyrate
CoA + N-acetyl-D-alpha-aminobutyrate
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?
acetyl-CoA + D-arginine
CoA + N-acetyl-D-arginine
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?
acetyl-CoA + D-asparaginate
CoA + N-acetyl-D-asparaginate
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ir
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
acetyl-CoA + D-cysteine
CoA + N-acetyl-D-cysteine
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ir
acetyl-CoA + D-cystine
CoA + N-acetyl-D-cystine
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ir
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
acetyl-CoA + D-histidine
CoA + N-acetyl-D-histidine
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ir
acetyl-CoA + D-isoleucine
CoA + N-acetyl-D-isoleucine
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ir
acetyl-CoA + D-leucine
CoA + N-acetyl-D-leucine
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ir
acetyl-CoA + D-lysine
CoA + N-acetyl-D-lysine
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?
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
acetyl-CoA + D-norleucine
CoA + N-acetyl-D-norleucine
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?
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
acetyl-CoA + D-proline
CoA + N-acetyl-D-proline
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ir
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
acetyl-CoA + D-tyrosine
CoA + N-acetyl-D-tyrosine
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ir
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
acetyl-CoA + norleucine
CoA + N-acetyl-D-norleucine
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ir
propionyl-CoA + D-alanine
CoA + N-propionyl-D-alanine
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ir
propionyl-CoA + D-allo-isoleucine
CoA + N-propionyl-D-allo-isoleucine
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ir
propionyl-CoA + D-glutamine
CoA + N-propionyl-D-glutamine
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ir
propionyl-CoA + D-leucine
CoA + N-propionyl-D-leucine
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ir
propionyl-CoA + D-serine
CoA + N-propionyl-D-serine
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ir
propionyl-CoA + D-tryptophan
CoA + N2-propionyl-D-tryptophan
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ir
additional information
?
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acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
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?
acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
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substrate specificity, overview
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acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
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D-amino acids are toxic to Saccharomyces cerevisiae but are detoxified with D-amino acid-N-acetyltransferase by N-acetylation preceding removal from yeast cells
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?
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
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?
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
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ir
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
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?
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
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ir
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
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?
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
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ir
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
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?
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
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ir
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
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?
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
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ir
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
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?
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
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ir
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
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?
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
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ir
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
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?
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
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ir
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
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?
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
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ir
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
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?
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
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ir
additional information
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L-isomers are not accepted, glycine and D-taurine are poor substrates, propionyl-CoA can replace acetyl-CoA
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?
additional information
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no activity with L-lysine, D-proline, and D-aspartate, D-glutamate is a poor substrate
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?
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acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
acetyl-CoA + D-alanine
CoA + N-acetyl-D-alanine
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ir
acetyl-CoA + D-asparaginate
CoA + N-acetyl-D-asparaginate
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ir
acetyl-CoA + D-asparagine
CoA + N-acetyl-D-asparagine
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ir
acetyl-CoA + D-cysteine
CoA + N-acetyl-D-cysteine
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ir
acetyl-CoA + D-cystine
CoA + N-acetyl-D-cystine
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ir
acetyl-CoA + D-glutamine
CoA + N-acetyl-D-glutamine
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ir
acetyl-CoA + D-histidine
CoA + N-acetyl-D-histidine
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ir
acetyl-CoA + D-isoleucine
CoA + N-acetyl-D-isoleucine
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ir
acetyl-CoA + D-leucine
CoA + N-acetyl-D-leucine
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ir
acetyl-CoA + D-methionine
CoA + N-acetyl-D-methionine
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ir
acetyl-CoA + D-norvaline
CoA + N-acetyl-D-norvaline
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ir
acetyl-CoA + D-phenylalanine
CoA + N-acetyl-D-phenylalanine
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ir
acetyl-CoA + D-proline
CoA + N-acetyl-D-proline
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ir
acetyl-CoA + D-serine
CoA + N-acetyl-D-serine
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ir
acetyl-CoA + D-threonine
CoA + N-acetyl-D-threonine
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ir
acetyl-CoA + D-tryptophan
CoA + N-acetyl-D-tryptophan
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ir
acetyl-CoA + D-tyrosine
CoA + N-acetyl-D-tyrosine
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ir
acetyl-CoA + D-valine
CoA + N-acetyl-D-valine
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ir
acetyl-CoA + norleucine
CoA + N-acetyl-D-norleucine
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ir
propionyl-CoA + D-alanine
CoA + N-propionyl-D-alanine
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ir
propionyl-CoA + D-allo-isoleucine
CoA + N-propionyl-D-allo-isoleucine
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ir
propionyl-CoA + D-glutamine
CoA + N-propionyl-D-glutamine
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ir
propionyl-CoA + D-leucine
CoA + N-propionyl-D-leucine
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ir
propionyl-CoA + D-serine
CoA + N-propionyl-D-serine
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ir
propionyl-CoA + D-tryptophan
CoA + N2-propionyl-D-tryptophan
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ir
acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
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?
acetyl-CoA + a D-amino acid
CoA + an N-acetyl-D-amino acid
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D-amino acids are toxic to Saccharomyces cerevisiae but are detoxified with D-amino acid-N-acetyltransferase by N-acetylation preceding removal from yeast cells
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?
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(NH4)2SO4
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strong inhibitor at 1 mM
CoA
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competitive against acetyl-CoA, noncompetitive against D-tryptophan
o-Iodosobenzoate
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strong inhibitor at 1 mM
p-chloromercuribenzoate
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strong inhibitor at 1 mM
ZnCl2
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strong inhibitor at 1 mM
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Brucellosis
The immunomodulatory effect of antimicrobial peptide HPA3P restricts Brucella abortus 544 infection in BALB/c mice.
Colonic Neoplasms
Antitumor activity of HPA3P through RIPK3-dependent regulated necrotic cell death in colon cancer.
Granuloma
The immunomodulatory effect of antimicrobial peptide HPA3P restricts Brucella abortus 544 infection in BALB/c mice.
Infections
The immunomodulatory effect of antimicrobial peptide HPA3P restricts Brucella abortus 544 infection in BALB/c mice.
Neoplasms
Antitumor activity of HPA3P through RIPK3-dependent regulated necrotic cell death in colon cancer.
Neoplasms
The immunomodulatory effect of antimicrobial peptide HPA3P restricts Brucella abortus 544 infection in BALB/c mice.
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0.054
D-alanine
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30°C, recombinant enzyme
0.034
D-serine
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30°C, recombinant enzyme
additional information
additional information
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kinetics
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0.227
acetyl-CoA
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4.5
D-tryptophan
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additional information
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substrate specificity
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7.3 - 7.5
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in phosphate buffer
8.4
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40
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brenda
gene HPA3
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brenda
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HPA3_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
161
0
18814
Swiss-Prot
other Location (Reliability: 1 )
A0A383RN23_9PSED
148
0
16771
TrEMBL
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A0A380STX6_9PSED
148
0
16921
TrEMBL
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A0A1D8N8L2_YARLL
150
0
17459
TrEMBL
other Location (Reliability: 1 )
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18000
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x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE
21000
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x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE
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?
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x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE
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-15°C, stable for several months
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native enzyme from dry yeast, to homogeneity in several steps, recombinant enzyme from Escherichia coli in a procedure similar to the purification of the native enzyme to 95% purity
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to homogeneity by ammonium sulfate fractionation, DEAE ion exchange chromatography, chromatography on a butyl resin, gel filtration, and D-glutamate affinity chromatography, overview
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gene HPA3, functional overexpression in Escherichia coli
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when the gene is overexpressed with a vector plasmid p426Gal1 in the wild type Saccharomyces cerevisiae as a host, the recombinant yeast, which shows more than 100 times higher D-amino acid-N-acetyltransferase activity than the wild type, is much more tolerant to D-amino acids than the wild type
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Zenk, M.H.; Schmitt, J.H.
Purification and properties of acetyl-CoA: D-amino acid-alpha-N-acetyltransferase from yeast
Biochem. Z.
342
54-65
1965
Saccharomyces cerevisiae
brenda
Yow, G.Y.; Uo, T.; Yoshimura, T.; Esaki, N.
D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids
Arch. Microbiol.
182
396-403
2004
Saccharomyces cerevisiae
brenda
Tashiro, Y.; Tamura, T.; Tanaka, H.; Inagaki, K.
Purification and some properties of D-amino acid acetyltransferase from Saccharomyces cerevisiae
Okayama Daigaku Nogakubu Gakujutsu Hokoku
93
13-18
2004
Saccharomyces cerevisiae
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brenda
Yow, G.Y.; Uo, T.; Yoshimura, T.; Esaki, N.
Physiological role of D-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of D-amino acids
Arch. Microbiol.
185
39-46
2006
Saccharomyces cerevisiae
brenda
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2.3.1.36 D-amino-acid N-acetyltransferase
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