Information on EC 2.3.1.3 - glucosamine N-acetyltransferase

for references in articles please use BRENDA:EC2.3.1.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)

The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
2.3.1.3
-
top print hide
RECOMMENDED NAME
GeneOntology No.
glucosamine N-acetyltransferase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + D-glucosamine = CoA + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:D-glucosamine N-acetyltransferase
-
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
9032-94-4
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Pigeon
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + chitobiose
CoA + N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
show the reaction diagram
-
77.5% activity compared to D-glucosamine
-
-
?
acetyl-CoA + chitopentaose
?
show the reaction diagram
-
4.7% activity compared to D-glucosamine
-
-
?
acetyl-CoA + chitotetraose
?
show the reaction diagram
-
9.3% activity compared to D-glucosamine
-
-
?
acetyl-CoA + chitotriose
?
show the reaction diagram
-
12% activity compared to D-glucosamine
-
-
?
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
acetyl-CoA + galactosamine
CoA + N-acetyl-D-galactosamine
show the reaction diagram
Pigeon
-
-
-
-
?
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
-
Cu2+
-
-
EDTA
-
1 mM EDTA inhibits enzyme by 20%
Fe2+
-
-
Fe3+
-
-
Zn2+
-
-
additional information
-
not: Mg2+, Mn2+, Ca2+, mono-, di and tri-phosphates of uridine, cytosine and tymidine
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 0.26
D-glucosamine
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Pigeon
-
-
Manually annotated by BRENDA team
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
PDB
SCOP
CATH
UNIPROT
ORGANISM
V9M3A9
Rhizomucor miehei;
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 1.3-1.6 M (NH4)2SO4, 0.1 M Bis-Tris pH (5.5–6.5) at 20°C
-
top print hide
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
CHAPS and digitonin solubilize 50% of enzyme activity
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-Gold (DE3) cells
-
top print hide
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H842A
-
inactive
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)