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Information on EC 2.3.1.277 - 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate synthase and Organism(s) Streptomyces griseus and UniProt Accession P18394

for references in articles please use BRENDA:EC2.3.1.277
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IUBMB Comments
The enzyme catalyses the first committed step in the biosynthesis of gamma-butyrolactone autoregulators that control secondary metabolism and morphological development in Streptomyces bacteria.
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This record set is specific for:
Streptomyces griseus
UNIPROT: P18394
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The taxonomic range for the selected organisms is: Streptomyces griseus
The expected taxonomic range for this enzyme is: Streptomyces
Reaction Schemes
a medium-chain 3-oxoacyl-[acyl-carrier protein]
+
=
2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate
+
Synonyms
afsA, barX, scbA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
afsA
-
-
-
-
barX
-
-
-
-
scbA
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
3-oxoacyl-[acyl-carrier protein]:glycerone phosphate 3-oxonacylltransferase
The enzyme catalyses the first committed step in the biosynthesis of gamma-butyrolactone autoregulators that control secondary metabolism and morphological development in Streptomyces bacteria.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate
2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate
2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
no metals are required for the activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
EDTA (5 mM) has no effect on the reaction
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2046
glycerone phosphate
pH 7.5, 15°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
glycerone phosphate
pH 7.5, 15°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
Trisx01HCl buffer
7 - 8
sodium phosphate-citrate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 30
at 30°C, the activity decreases to 32% of that measured at 15°C, and almost no activity is detected at 35°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-y-butyrolactone) biosynthesis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AFSA_STRGR
301
0
32706
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45400
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 37700, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kato, J.; Funa, N.; Watanabe, H.; Ohnishi, Y.; Horinouchi, S.
Biosynthesis of gamma-butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces
Proc. Natl. Acad. Sci. USA
104
2378-2383
2007
Streptomyces griseus (P18394), Streptomyces griseus IFO13350 (P18394)
-
Manually annotated by BRENDA team
Horinouchi, S.; Suzuki, H.; Nishiyama, M.; Beppu, T.
Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis
J. Bacteriol.
171
1206-1210
1989
Streptomyces griseus (P18394)
Manually annotated by BRENDA team