Literature summary for 2.3.1.277 extracted from

Kato, J.; Funa, N.; Watanabe, H.; Ohnishi, Y.; Horinouchi, S.
Biosynthesis of gamma-butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces (2007), Proc. Natl. Acad. Sci. USA, 104, 2378-2383 .

No PubMed abstract available

Search for more literature for 2.3.1.277

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces griseus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	EDTA (5 mM) has no effect on the reaction	Streptomyces griseus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2046	-	glycerone phosphate	pH 7.5, 15°C	Streptomyces griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no metals are required for the activity	Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37700	-	SDS-PAGE	Streptomyces griseus
45400	-	gel filtration	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	Streptomyces griseus	AfsA is the key enzyme for A factor biosynthesis	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	Streptomyces griseus IFO13350	AfsA is the key enzyme for A factor biosynthesis	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	P18394	-	-
Streptomyces griseus IFO13350	P18394	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces griseus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	-	Streptomyces griseus	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	AfsA is the key enzyme for A factor biosynthesis	Streptomyces griseus	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	-	Streptomyces griseus IFO13350	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?
a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate	AfsA is the key enzyme for A factor biosynthesis	Streptomyces griseus IFO13350	2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein]	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 37700, SDS-PAGE	Streptomyces griseus

Synonyms

Synonyms	Comment	Organism
afsA	-	Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
15	-	-	Streptomyces griseus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	30	at 30°C, the activity decreases to 32% of that measured at 15°C, and almost no activity is detected at 35°C	Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	-	glycerone phosphate	pH 7.5, 15°C	Streptomyces griseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7.5	Trisx01HCl buffer	Streptomyces griseus
7	8	sodium phosphate-citrate buffer	Streptomyces griseus

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Release 2023.1 (January 2023)