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Information on EC 2.3.1.196 - benzyl alcohol O-benzoyltransferase and Organism(s) Nicotiana tabacum and UniProt Accession Q8GT20

for references in articles please use BRENDA:EC2.3.1.196
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IUBMB Comments
The enzyme is involved in volatile benzenoid and benzoic acid biosynthesis. The enzyme from Petunia hybrida also catalyses the formation of 2-phenylethyl benzoate from benzoyl-CoA and 2-phenylethanol. The apparent catalytic efficiency of the enzyme from Petunia hybrida with benzoyl-CoA is almost 6-fold higher than with acetyl-CoA .
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Nicotiana tabacum
UNIPROT: Q8GT20
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Word Map
The taxonomic range for the selected organisms is: Nicotiana tabacum
The enzyme appears in selected viruses and cellular organisms
Synonyms
phbpbt, hsr201, benzoyl-coa:benzyl alcohol/phenylethanol benzoyltransferase, benzyl alcohol o-benzoyltransferase, benzoyl-coa:benzyl alcohol o-benzoyltransferase, benzoyl coa:benzyl alcohol/phenylethanol benzoyltransferase, benzoyl-coa salicyl alcohol o-benzoyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
benzoyl-CoA:benzyl alcohol O-benzoyltransferase
The enzyme is involved in volatile benzenoid and benzoic acid biosynthesis. The enzyme from Petunia hybrida also catalyses the formation of 2-phenylethyl benzoate from benzoyl-CoA and 2-phenylethanol. The apparent catalytic efficiency of the enzyme from Petunia hybrida with benzoyl-CoA is almost 6-fold higher than with acetyl-CoA [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + benzyl alcohol
benzyl benzoate + CoA
show the reaction diagram
-
-
-
?
additional information
?
-
no activity with acetyl-CoA at concentrations below 1 mM
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
benzoyl-CoA
pH 7.7, 22°C, cosustrate: benzyl alcohol
0.019
benzyl alcohol
pH 7.7, 22°C, cosustrate: benzoyl-CoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
induced upon wounding
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BEBT_TOBAC
460
0
51014
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51010
calculated from sequence
51014
x * 51014, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 51014, calculated from sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
D'Auria, J.C.; Chen, F.; Pichersky, E.
Characterization of an acyltransferase capable of synthesizing benzyl benzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri
Plant Physiol.
130
466-476
2002
Clarkia breweri (Q8GT21), Nicotiana tabacum (Q8GT20)
Manually annotated by BRENDA team