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Information on EC 2.3.1.19 - phosphate butyryltransferase and Organism(s) Clostridium acetobutylicum and UniProt Accession P58255

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Clostridium acetobutylicum
UNIPROT: P58255 not found.
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The taxonomic range for the selected organisms is: Clostridium acetobutylicum
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
phosphotransbutyrylase, phosphate butyryltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phophotransbutyrylase
-
butyryltransferase, phosphate
-
-
-
-
phosphotransbutyrylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
butanoyl-CoA:phosphate butanoyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-01-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxybutanoyl-CoA + phosphate
3-hydroxybutanoylphosphate + CoA
show the reaction diagram
-
-
-
-
r
4-hydroxybutanoyl-CoA + phosphate
4-hydroxybutanoylphosphate + CoA
show the reaction diagram
-
-
-
-
r
4-hydroxypentanoyl-CoA + phosphate
4-hydroxypentanoylphosphate + CoA
show the reaction diagram
-
-
-
-
r
acetyl-CoA + phosphate
acetylphosphate + CoA
show the reaction diagram
-
1.3% of butanoylphosphate formation
-
-
?
butanoyl-CoA + phosphate
butanoylphosphate + CoA
show the reaction diagram
isobutanoyl-CoA + phosphate
isobutanoylphosphate + CoA
show the reaction diagram
-
30% of butanoylphosphate formation
-
-
?
isopentanoyl-CoA + phosphate
isopentanoylphosphate + CoA
show the reaction diagram
-
95% of butanoylphosphate formation
-
-
?
n-pentanoyl-CoA + phosphate
n-pentanoylphosphate + CoA
show the reaction diagram
-
78% of butanoylphosphate formation
-
-
?
n-propionyl-CoA + phosphate
n-propionylphosphate + CoA
show the reaction diagram
-
23% of butanoylphosphate formation
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
butanoyl-CoA
-
-
0.26
Butanoylphosphate
-
-
0.14
phosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
study on gene dosage effects
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
butanoylphosphate formation
8.8
-
butanoyl-CoA formation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 9.5
-
butanoylphosphate formation, less than 50% of maximal activity above and below
6.8 - 9.5
-
butanoyl-CoA formation, less than 50% of maximal activity above and below
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
a butanoate kinase mutant is not viable if the ptb gene is not also inactivated. In a butanoate kinase/phosphate butanoyltransferase double mutant under acidogenic conditions, the primary metabolite is butanol and 2-hydroxyvalerate is produced. Under solventogenesis, 58% increased butanol production is obtained compared to the control, and 0.3 g/g butanol formation is reached. Under alcohologenesis, the major product is lactate. AdhE2, which encodes an aldehyde/alcohol dehydrogenase, is highly expressed in all metabolic states in the mutant
physiological function
-
The product pattern of a gene disruption mutant is altered to high ethanol, i.e. 12.1 g/l, and high butanol, i.e. 8.0 g/l, titers in fermentations with pH regulated above 5.0. Glucose fed-batch cultivation elevates the ethanol concentration to 32.4 g/l, yielding a more than fourfold increased alcohol to acetone ratio as compared to the wildtype. The mutant is still capable to take up butanoate when externally added during the late exponential growth phase. Findings suggest that alternative pathways of butanoate re-assimilation exist in Clostridium acetobutylicum
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
264000
-
gel filtration
31000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 31000, SDS-PAGE
octamer
-
8 * 31000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the phosphotransbutyrylase promoter is used to produce butanol from butyryl-CoA with alcohol/aldehyde dehydrogenase
biotechnology
-
use of enzyme for in vitro biosynthesis of poly(hydroxyalkanoic acid)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wiesenborn, D.P.; Rudolph, F.B.; Papoutsakis, E.T.
Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and its role in acidogenesis
Appl. Environ. Microbiol.
55
317-322
1989
Clostridium acetobutylicum
Manually annotated by BRENDA team
Cary, J.W.; Petersen, D.J.; Papoutsakis, E.T.; Bennett, G.N.
Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli
J. Bacteriol.
170
4613-4618
1988
Clostridium acetobutylicum
Manually annotated by BRENDA team
Walter, K.A.; Mermelstein, L.D.; Papoutsakis, E.T.
Studies of recombinant Clostridium acetobutylicum with increased dosages of butyrate formation genes
Ann. N. Y. Acad. Sci.
721
69-72
1994
Clostridium acetobutylicum
Manually annotated by BRENDA team
Liu, S.J.; Steinbuchel, A.
Exploitation of butyrate kinase and phosphotransbutyrylase from Clostridium acetobutylicum for the in vitro biosynthesis of poly(hydroxyalkanoic acid)
Appl. Microbiol. Biotechnol.
53
545-552
2000
Clostridium acetobutylicum
Manually annotated by BRENDA team
Oultram, J.D.; Burr, I.D.; Elmore, M.J.; Minton, N.P.
Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052
Gene
131
107-112
1993
Clostridium acetobutylicum, Clostridium acetobutylicum NCIMB 8052
Manually annotated by BRENDA team
Sillers, R.; Al-Hinai, M.A.; Papoutsakis, E.T.
Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
Biotechnol. Bioeng.
102
38-49
2009
Clostridium acetobutylicum (P58255), Clostridium acetobutylicum
Manually annotated by BRENDA team
Lehmann, D.; Radomski, N.; Luetke-Eversloh, T.
New insights into the butyric acid metabolism of Clostridium acetobutylicum
Appl. Microbiol. Biotechnol.
96
1325-1339
2012
Clostridium acetobutylicum
Manually annotated by BRENDA team
Yoo, M.; Croux, C.; Meynial-Salles, I.; Soucaille, P.
Metabolic flexibility of a butyrate pathway mutant of Clostridium acetobutylicum
Metab. Eng.
40
138-147
2017
Clostridium acetobutylicum (P58255), Clostridium acetobutylicum, Clostridium acetobutylicum DSM 792 (P58255)
Manually annotated by BRENDA team