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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA]
=
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a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]
Synonyms
cap-dependent 2'-O-methyltransferase, messenger ribonucleate 2'-O-methyladenosine NG-methyltransferase, mRNA cap (nucleoside-2'O)-methyltransferase, NS5MTaseDV, VP39,
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cap-dependent 2'-O-methyltransferase
Variola sp.
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messenger ribonucleate 2'-O-methyladenosine NG-methyltransferase
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mRNA cap (nucleoside-2'O)-methyltransferase
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S-adenosyl-L-methionine + a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA] = S-adenosyl-L-homocysteine + a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]
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methyl group transfer
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S-adenosyl-L-methionine:mRNA (2'-O-methyladenosine-N6-)-methyltransferase
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S-adenosyl-L-methionine + 7MeGpppAC5
S-adenosyl-L-homocysteine + 7MeGpppA2'OMeC5
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RNA substrate is used
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?
S-adenosyl-L-methionine + G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA
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poor substrate
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S-adenosyl-L-methionine + m7G(5')pppA-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA
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?
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
S-adenosyl-L-methionine + pppA-mRNA
S-adenosyl-L-homocysteine + pppm6A-mRNA
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additional information
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no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA
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S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
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methyl group acceptor is methylated or unmethylated vaccinia virus RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
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S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
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involved in post-transcriptional modification of 5'-end of messenger-RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
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S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
Variola sp.
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also acts as poly(A) polymerase progressivity factor
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?
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S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
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involved in post-transcriptional modification of 5'-end of messenger-RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
?
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N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
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0.1 mM, 75% inhibition. It is suggested that the inhibitor competes with the cofactor AdoMet and presumably also the capped RNA substrate
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erythromycin
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enzyme synthesis induced by
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Dengue
Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2'O)-methyltransferase.
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0.0605
N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
Dengue virus
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brenda
human, HeLa S3 cells
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brenda
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brenda
Variola sp.
vaccinia virus
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brenda
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brenda
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65000
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sucrose density gradient
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monomer
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1 * 65000, SDS-PAGE
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-70°C, 0.21 M (NH4)2SO4, stable for longer than 5 months
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0°C, 0.21 M (NH4)2SO4, stable for 4-5 months
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ermC gene of pE194 specifies resistance to macrolide-lincosamide-streptogramin B antibiotics
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Keith, J.M.; Ensinger, M.J.; Moss, B.
HeLa cell RNA (2-O-methyladenosine-N6-)-methyltransferase specific for the capped 5-end of messenger RNA
J. Biol. Chem.
253
5033-5041
1978
Homo sapiens
brenda
Shivakumar, A.G.; Dubnau, D.
Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance
Nucleic Acids Res.
9
2549-2562
1981
Staphylococcus aureus
brenda
Shi, X.; Yao, P.; Jose, T.; Gershon, P.D.
Methyltransferase-specific domains within VP39, a bifunctional protein that participates in the modification of both mRNA ends
RNA
2
88-101
1996
Variola sp.
brenda
Luzhkov, V.B.; Selisko, B.; Nordqvist, A.; Peyrane, F.; Decroly, E.; Alvarez, K.; Karlen, A.; Canard, B.; Qvist, J.
Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2O)-methyltransferase
Bioorg. Med. Chem.
15
7795-7802
2007
Dengue virus
brenda
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2.1.1.62 mRNA (2'-O-methyladenosine-N6-)-methyltransferase
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