Information on EC 2.1.1.62 - mRNA (2'-O-methyladenosine-N6-)-methyltransferase

for references in articles please use BRENDA:EC2.1.1.62
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.1.1.62
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RECOMMENDED NAME
GeneOntology No.
mRNA (2'-O-methyladenosine-N6-)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am (mRNA containing an N6,2'-O-dimethyladenosine cap)
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:mRNA (2'-O-methyladenosine-N6-)-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
68009-87-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
human, HeLa S3 cells
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Manually annotated by BRENDA team
Variola sp.
vaccinia virus
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7MeGpppAC5
S-adenosyl-L-homocysteine + 7MeGpppA2'OMeC5
show the reaction diagram
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RNA substrate is used
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-
?
S-adenosyl-L-methionine + G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA
show the reaction diagram
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poor substrate
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S-adenosyl-L-methionine + m7G(5')pppA-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA
show the reaction diagram
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-
-
-
?
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
show the reaction diagram
S-adenosyl-L-methionine + pppA-mRNA
S-adenosyl-L-homocysteine + pppm6A-mRNA
show the reaction diagram
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?
additional information
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no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
show the reaction diagram
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involved in post-transcriptional modification of 5'-end of messenger-RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AdoMet
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
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0.1 mM, 75% inhibition. It is suggested that the inhibitor competes with the cofactor AdoMet and presumably also the capped RNA substrate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
erythromycin
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enzyme synthesis induced by
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0605
N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
Dengue virus
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000418
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
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sucrose density gradient
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 65000, SDS-PAGE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 0.21 M (NH4)2SO4, stable for longer than 5 months
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0°C, 0.21 M (NH4)2SO4, stable for 4-5 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ermC gene of pE194 specifies resistance to macrolide-lincosamide-streptogramin B antibiotics
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Show AA Sequence (115 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.62)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)