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Information on EC 2.1.1.62 - mRNA (2'-O-methyladenosine-N6-)-methyltransferase

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EC Tree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.62 mRNA (2'-O-methyladenosine-N6-)-methyltransferase

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The enzyme appears in viruses and cellular organisms

Reaction Schemes

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S-adenosyl-L-methionine

a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA]

S-adenosyl-L-homocysteine

a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA]

a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

Synonyms

ns5mtasedv, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

cap-dependent 2'-O-methyltransferase

Variola sp.

485485

messenger ribonucleate 2'-O-methyladenosine NG-methyltransferase

mRNA cap (nucleoside-2'O)-methyltransferase

NS5MTaseDV

VP39

Variola sp.

485485

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

S-adenosyl-L-methionine + a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA] = S-adenosyl-L-homocysteine + a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

methyl group transfer

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SYSTEMATIC NAME

IUBMB Comments

S-adenosyl-L-methionine:mRNA (2'-O-methyladenosine-N6-)-methyltransferase

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CAS REGISTRY NUMBER

COMMENTARY

68009-87-0

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + 7MeGpppAC5

S-adenosyl-L-homocysteine + 7MeGpppA2'OMeC5

Dengue virus

RNA substrate is used

685528

S-adenosyl-L-methionine + G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA

Homo sapiens

poor substrate

485483

S-adenosyl-L-methionine + m7G(5')pppA-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA

Homo sapiens

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

3 entries

S-adenosyl-L-methionine + pppA-mRNA

S-adenosyl-L-homocysteine + pppm6A-mRNA

Homo sapiens

485483

additional information

Homo sapiens

no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

methyl group acceptor is methylated or unmethylated vaccinia virus RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

involved in post-transcriptional modification of 5'-end of messenger-RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Variola sp.

also acts as poly(A) polymerase progressivity factor

485485

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

involved in post-transcriptional modification of 5'-end of messenger-RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

AdoMet

Dengue virus

685528

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

Dengue virus

0.1 mM, 75% inhibition. It is suggested that the inhibitor competes with the cofactor AdoMet and presumably also the capped RNA substrate

685528

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

erythromycin

Staphylococcus aureus

enzyme synthesis induced by

485484

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Dengue

Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2'O)-methyltransferase.

17888664

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IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0605

N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

Dengue virus

685528

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.000418

Homo sapiens

485483

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

human, HeLa S3 cells

brenda

Staphylococcus aureus

485484

brenda

Variola sp.

vaccinia virus

485485

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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PDB

SCOP

CATH

UNIPROT

ORGANISM

16 entries

Homo sapiens

5il0, download, 3D-view

5il1, download, 3D-view

5il2, download, 3D-view

5k7m, download, 3D-view

5k7u, download, 3D-view

5k7w, download, 3D-view

5l6d, download, 3D-view

5l6e, download, 3D-view

5tey, download, 3D-view

6b91, download, 3D-view

6b92, download, 3D-view

6du4, download, 3D-view

6du5, download, 3D-view

6gfk, download, 3D-view

6gfn, download, 3D-view

6gt5, download, 3D-view

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

29000

Staphylococcus aureus

PAGE

485484

65000

Homo sapiens

sucrose density gradient

485483

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

monomer

Homo sapiens

1 * 65000, SDS-PAGE

485483

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-70°C, 0.21 M (NH4)2SO4, stable for longer than 5 months

Homo sapiens

485483

0°C, 0.21 M (NH4)2SO4, stable for 4-5 months

Homo sapiens

485483

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Staphylococcus aureus

partially

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

ermC gene of pE194 specifies resistance to macrolide-lincosamide-streptogramin B antibiotics

Staphylococcus aureus

485484

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

485483

Keith, J.M.; Ensinger, M.J.; Moss, B.

HeLa cell RNA (2-O-methyladenosine-N6-)-methyltransferase specific for the capped 5-end of messenger RNA

J. Biol. Chem.

253

5033-5041

1978

Homo sapiens

670176

brenda

485484

Shivakumar, A.G.; Dubnau, D.

Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance

Nucleic Acids Res.

2549-2562

1981

Staphylococcus aureus

6792593

brenda

485485

Shi, X.; Yao, P.; Jose, T.; Gershon, P.D.

Methyltransferase-specific domains within VP39, a bifunctional protein that participates in the modification of both mRNA ends

RNA

88-101

1996

Variola sp.

8846300

brenda

685528

Luzhkov, V.B.; Selisko, B.; Nordqvist, A.; Peyrane, F.; Decroly, E.; Alvarez, K.; Karlen, A.; Canard, B.; Qvist, J.

Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2O)-methyltransferase

Bioorg. Med. Chem.

7795-7802

2007

Dengue virus

17888664

brenda

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EXTERNAL LINKS (specific for EC-Number 2.1.1.62)

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

IUBMB Enzyme Nomenclature

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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