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Information on EC 2.1.1.62 - mRNA (2'-O-methyladenosine-N6-)-methyltransferase

for references in articles please use BRENDA:EC2.1.1.62

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EC Tree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.62 mRNA (2'-O-methyladenosine-N6-)-methyltransferase

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The enzyme appears in viruses and cellular organisms

Reaction Schemes

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S-adenosyl-L-methionine

a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA]

S-adenosyl-L-homocysteine

a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA]

a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

Synonyms

ns5mtasedv, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

cap-dependent 2'-O-methyltransferase

Variola sp.

485485

messenger ribonucleate 2'-O-methyladenosine NG-methyltransferase

mRNA cap (nucleoside-2'O)-methyltransferase

NS5MTaseDV

VP39

Variola sp.

485485

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

S-adenosyl-L-methionine + a 5-(N7-methyl 5-triphosphoguanosine)-2'-O-methyladenosine-[mRNA] = S-adenosyl-L-homocysteine + a 5-(N7-methyl 5-triphosphoguanosine)-N6,2'-O-dimethyladenosine-[mRNA]

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

methyl group transfer

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SYSTEMATIC NAME

IUBMB Comments

S-adenosyl-L-methionine:mRNA (2'-O-methyladenosine-N6-)-methyltransferase

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CAS REGISTRY NUMBER

COMMENTARY

68009-87-0

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + 7MeGpppAC5

S-adenosyl-L-homocysteine + 7MeGpppA2'OMeC5

Dengue virus

RNA substrate is used

685528

S-adenosyl-L-methionine + G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA

Homo sapiens

poor substrate

485483

S-adenosyl-L-methionine + m7G(5')pppA-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA

Homo sapiens

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

3 entries

S-adenosyl-L-methionine + pppA-mRNA

S-adenosyl-L-homocysteine + pppm6A-mRNA

Homo sapiens

485483

additional information

Homo sapiens

no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

methyl group acceptor is methylated or unmethylated vaccinia virus RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

involved in post-transcriptional modification of 5'-end of messenger-RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Variola sp.

also acts as poly(A) polymerase progressivity factor

485485

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + m7G(5')pppAm-mRNA

S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA

Homo sapiens

involved in post-transcriptional modification of 5'-end of messenger-RNA

485483

mRNA containing an N6,2'-O-dimethyladenosine cap

485483

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

AdoMet

Dengue virus

685528

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

Dengue virus

0.1 mM, 75% inhibition. It is suggested that the inhibitor competes with the cofactor AdoMet and presumably also the capped RNA substrate

685528

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

erythromycin

Staphylococcus aureus

enzyme synthesis induced by

485484

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Dengue

Virtual screening and bioassay study of novel inhibitors for dengue virus mRNA cap (nucleoside-2'O)-methyltransferase.

17888664

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IC50 VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0605

N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

Dengue virus

685528

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.000418

Homo sapiens

485483

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

human, HeLa S3 cells

brenda

Staphylococcus aureus

485484

brenda

Variola sp.

vaccinia virus

485485

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

P59114 pBLAST

CAPAM_MOUSE

Mus musculus

706

80504

Swiss-Prot