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Information on EC 2.1.1.45 - thymidylate synthase and Organism(s) Pneumocystis carinii and UniProt Accession P13100

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase
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This record set is specific for:
Pneumocystis carinii
UNIPROT: P13100 not found.
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The taxonomic range for the selected organisms is: Pneumocystis carinii
The enzyme appears in selected viruses and cellular organisms
Synonyms
thymidylate synthase, thymidylate synthetase, human ts, dtmp synthase, thymidylate synthase a, tmp synthetase, y110a7a.4, 5,10-methylenetetrahydrofolate:dump c-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dTMP synthase
-
-
-
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methylenetetrahydrofolate:dUMP C-methyltransferase
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-
-
-
thymidylate synthetase
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-
-
-
TMP synthetase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehalogenation
-
-
-
-
methylene group transfer
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-
-
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reductive methylation
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:dUMP C-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-61-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism
-
-
?
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
-
biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N5,N10-methylenetetrahydrofolate
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-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1-bis-(4-hydroxyphenyl)-1H-naphtho[1,2-c]furan-3-one
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-
3,3-bis-(3-chloro-4-hydroxyphenyl)-1H,3H-[1,8-c,d]pyran-1-one
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benzyloxycarbonylamino-N,O-didansyl-L-tyrosine
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N,O-didansyl-L-tyrosine
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TYSY_PNECA
297
0
34362
Swiss-Prot
other Location (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals belong to space group P2(1)2(1)2(1), a = 54.05 A, b = 66.16 A and c = 178.76 A
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Livi, L.L.; Edman, U.; Schneider, G.P.; Greene, P.J.; Santi, D.V.
Cloning, expression and characterization of thymidylate synthase from Cryptococcus neoformans
Gene
150
221-226
1994
Tequatrovirus T4, Candida albicans, Homo sapiens, Lacticaseibacillus casei, Mus musculus, Pneumocystis carinii, Cryptococcus neoformans (P0CS13), Cryptococcus neoformans, Cryptococcus neoformans B-3501A (P0CS13)
Manually annotated by BRENDA team
Anderson, A.C.; O'Neil, R.H.; DeLano, W.L.; Stroud, R.M.
The structural mechanism for half-the-sites reactivity in an enzyme, thymidylate synthase, involves a relay of changes between subunits
Biochemistry
38
13829-13836
1999
Pneumocystis carinii (P13100), Pneumocystis carinii
Manually annotated by BRENDA team
Anderson, A.C.; Perry, K.M.; Freymann, D.M.; Stroud, R.M.
The crystal structure of thymidylate synthase from Pneumocystis carinii reveals a fungal insert important for drug design
J. Mol. Biol.
297
645-657
2000
Pneumocystis carinii
Manually annotated by BRENDA team
Golos, B.; Dzik, J.M.; Kazimierczuk, Z.; Ciesla, J.; Zielinski, Z.; Jankowska, J.; Kraszewski, A.; Stawinski, J.; Rode, W.; Shugar, D.
Interaction of thymidylate synthase with the 5'-thiophosphates, 5'-dithiophosphates, 5'-H-phosphonates and 5'-S-thiosulfates of 2'-deoxyuridine, thymidine and 5-fluoro-2'-deoxyuridine
Biol. Chem.
382
1439-1445
2001
Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus, Pneumocystis carinii, Rattus norvegicus, Trichinella pseudospiralis, Trichinella spiralis
Manually annotated by BRENDA team
Saxl, R.L.; Changchien, L.M.; Hardy, L.W.; Maley, F.
Parameters affecting the restoration of activity to inactive mutants of thymidylate synthase via subunit exchange: Further evidence that thymidylate synthase Is a half-of-the-sites activity enzyme
Biochemistry
40
5275-5282
2001
Escherichia coli, Lacticaseibacillus casei, Pneumocystis carinii
Manually annotated by BRENDA team
Ferrari, S.; Losasso, V.; Costi, M.
Sequence-based identification of specific drug target regions in the thymidylate synthase enzyme family
ChemMedChem
3
392-401
2008
Acinetobacter baumannii, Aeromonas hydrophila, Bordetella bronchiseptica, Ascaris suum, Aspergillus clavatus, Aspergillus fischeri, Aspergillus fumigatus, Aspergillus nidulans, Bacillus amyloliquefaciens, Bacillus anthracis, Bacillus cereus, Bacillus licheniformis, Bacillus mycoides, Bacteroides thetaiotaomicron, Bacteroides fragilis, Porphyromonas gingivalis, Bartonella bacilliformis, Bordetella parapertussis, Bordetella pertussis, Brucella abortus, Brucella melitensis, Burkholderia mallei, Burkholderia pseudomallei, Candida albicans, Debaryomyces hansenii, [Candida] glabrata, Chaetomium globosum, Chromobacterium violaceum, Clostridioides difficile, Coccidioides immitis, Comamonas testosteroni, Corynebacterium diphtheriae, Coxiella burnetii, Cryptococcus neoformans, Cryptosporidium parvum, Streptococcus pneumoniae, Enterobacter sp., Enterococcus faecalis, Enterococcus faecium, Salmonella enterica, Francisella tularensis, Fusobacterium nucleatum, [Haemophilus] ducreyi, Haemophilus influenzae, Human alphaherpesvirus 1, Legionella pneumophila, Leishmania amazonensis, Leishmania braziliensis, Leishmania donovani, Leishmania infantum, Leishmania major, Leishmania tropica, Listeria monocytogenes, Staphylococcus aureus, Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium tuberculosis variant bovis, Mycobacterium leprae, Mycoplasma genitalium, Mycoplasma pneumoniae, Neisseria gonorrhoeae, Neisseria meningitidis, Pasteurella multocida, Yersinia pestis, Yersinia pseudotuberculosis, Plasmodium vivax, Pneumocystis carinii, Cutibacterium acnes, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Pseudomonas mendocina, Shewanella putrefaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Paratyphi A, Salmonella enterica subsp. enterica serovar Typhi, Schistosoma japonicum, Shigella boydii, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Novosphingobium aromaticivorans, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Streptococcus agalactiae, Streptococcus pyogenes, Streptococcus mutans, Streptococcus sanguinis, Toxoplasma gondii, Trichinella spiralis, Trypanosoma brucei, Trypanosoma cruzi, Vibrio alginolyticus, Vibrio parahaemolyticus, Yersinia enterocolitica, Plasmodium malariae, Cryptosporidium hominis, Encephalitozoon cuniculi, Vibrio vulnificus, Bartonella henselae, Bartonella quintana, Mycobacterium ulcerans, Burkholderia multivorans, Yersinia bercovieri, Yersinia frederiksenii, Brucella suis, Serratia proteamaculans, Yersinia intermedia, Burkholderia vietnamiensis, Nocardia farcinica, Malacoplasma penetrans, Burkholderia ambifaria, Corynebacterium jeikeium, Yersinia mollaretii, Burkholderia dolosa, Vibrio cholerae serotype O1, Plasmodium falciparum (P13922)
Manually annotated by BRENDA team