Ligand 7,8-dihydrofolate

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Basic Ligand Information

Molecular Structure
Picture of 7,8-dihydrofolate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C19H21N7O6
7,8-dihydrofolate
OZRNSSUDZOLUSN-LBPRGKRZSA-N
Synonyms:
7,8-dihydropteroyl-Glu, 7,8-dihydropteroylglutamate, dihydrofolate, dihydrofolate monoglutamate, dihydrofolic acid, dihydropteroyl-Glu, dihydropteroyl-L-Glu, dihydropteroylglutamate, N-(4-{[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid


Show all pahtways known for Show all BRENDA pathways known for 7,8-dihydrofolate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (30 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
folate + NADPH + H+ = 7,8-dihydrofolate + tetrahydrofolate + NADP+
show the reaction diagram
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5,10-methylenetetrahydrofolate + dCMP = dihydrofolate + deoxy-5-methylcytidylate
show the reaction diagram
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-
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram
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2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
show the reaction diagram
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ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
show the reaction diagram
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-

Substrate in Enzyme-catalyzed Reactions (69 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
Dihydrofolate + NADPH = 5,6,7,8-Tetrahydrofolate + NADP+
show the reaction diagram
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dihydrofolate + NADPH = tetrahydrofolate + NADP+
show the reaction diagram
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7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
show the reaction diagram
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dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
show the reaction diagram
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dihydrofolic acid + H2O = dihydropteroic acid + Glu
show the reaction diagram
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ATP + formate + dihydrofolate = ADP + phosphate + 10-formyldihydrofolate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (77 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
folate + NADPH + H+ = 7,8-dihydrofolate + tetrahydrofolate + NADP+
show the reaction diagram
-
-
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activates
-

Inhibitor in Enzyme-catalyzed Reactions (47 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive inhibitor with respect to tetrahydrofolate
-
weak
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3D Structure of Enzyme-Ligand-Complex (PDB) (130 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (204 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.84
-
-

KM Value (242 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.04
-
cosubstrate NADPH, pH 7.2, 25°C

Ki Value (7 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0065
-
-
0.006
-
pH 6.7, 25°C
0.021
-
-

References & Links

Links to other databases for 7,8-dihydrofolate

ChEBI
PubChem
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PubChem