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Ligand 7,8-dihydrofolate
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Basic Ligand Information
7,8-dihydropteroyl-Glu, 7,8-dihydropteroylglutamate, dihydrofolate, dihydrofolate monoglutamate, dihydrofolic acid, dihydropteroyl-Glu, dihydropteroyl-L-Glu, dihydropteroylglutamate, N-(4-{[(2-amino-4-oxo-3,4,7,8-tetrahydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid
KEGG
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Show all BRENDA pathways known for 7,8-dihydrofolate
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open all tablesRoles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (30 results)
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EC NUMBER 
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 693567, 705044, 724168, 724390, 702667, 703158, 705018, 705800, 726365, 723808, 724311, 724540, 703160, 703329, 702275, 705016, 724750, 724293, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 742057, 701696, 724416, 725204, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 705735, 757625
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
In Vivo Product in Enzyme-catalyzed Reactions (23 results)
EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
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5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
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5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
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10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
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2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
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ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
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Substrate in Enzyme-catalyzed Reactions (69 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + acetylpyridine adenine nucleotide, reduced = 5,6,7,8-tetrahydrofolate + acetylpyridine adenine nucleotide, oxidized
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADH + H+ = 5,6,7,8-tetrahydrofolate + NAD+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADP + H+ = 5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH + H+ = 5,6,7,8-tetrahydrofolate + NADP+
743741, 685247, 693567, 705044, 724168, 724390, 747067, 686360, 702667, 703158, 705018, 705800, 726365, 723808, 392252, 705735, 724311, 392241, 724540, 684488, 687184, 688889, 703160, 703329, 392250, 702275, 705016, 688027, 724750, 673502, 392229, 724293, 688381, 689962, 703803, 741536, 741613, 741615, 742518, 743758, 741889, 743663, 690467, 701734, 705405, 725661, 705138, 706654, 686658, 742057, 685386, 687680, 701696, 724416, 725204, 757625, 742438, 742491, 743678, 725924, 741552, 741892, 743569, 742074, 747064, 701473
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
7,8-dihydrofolate + NADPH = 5,6,7,8-tetrahydrofolate + NADP+
392243, 392207, 392208, 392210, 392224, 392274, 671364, 392255, 392290, 392268, 392211, 392263, 392212, 392277, 392286, 654970, 674351, 392260, 392288, 655633, 687840, 392281, 392269, 392275, 672035, 392267, 671970, 392244, 392245, 392285, 392284, 392291, 392229, 392230, 392237, 392253, 392215, 392242, 392221, 392227, 392235, 392236, 654118, 392295, 392293, 392294, 392283, 673502, 392254, 392289, 676947, 676933, 674549, 655861, 673457, 654202, 392292, 392248, 392259, 392271, 392280, 392232, 392249, 654526, 655867, 656214, 657316
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
dihydrofolate + NADPH + H+ = tetrahydrofolate + NADP+
Dihydrofolate + NADPH = 5,6,7,8-Tetrahydrofolate + NADP+
-
ADP + phosphate + 7,8-dihydropteroyl-Glu = ATP + 7,8-dihydropteroate + L-glutamate
-
ATP + 7,8-dihydropteroyl-Glu + L-glutamate = ADP + phosphate + 7,8-dihydropteroyl-gamma-Glu2
-
ATP + 7,8-dihydropteroyl-Glu + L-glutamate = ADP + phosphate + 7,8-dihydropteroyl-Glu2
-
Product in Enzyme-catalyzed Reactions (77 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate = 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate
-
(6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate = 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate
-
(6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate = 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate
-
(R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
-
(R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
-
(R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
-
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate = 7,8-dihydrofolate + ?
-
5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
-
5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
-
5,10-methylenetetrahydrofolate + deoxyuridine monophosphate = 7,8-dihydrofolate + deoxythymidine monophosphate
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-dithiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-dithiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-dithiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-thiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-thiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 2'-deoxyuridine 5'-thiophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 5-hydroxy-2'-deoxyuridine 5'-monophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 5-hydroxy-2'-deoxyuridine 5'-monophosphate = 7,8-dihydrofolate + ?
-
N5,10-methylenetetrahydrofolate + 5-hydroxy-2'-deoxyuridine 5'-monophosphate = 7,8-dihydrofolate + ?
-
(-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
(-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
(-)-tetrahydropteroylglutamate + 2'-deoxyuridine 5'-monophosphate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
(6S)-tetrahydropteroylglutamate + 2-deoxyuridylate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
(6S)-tetrahydropteroylglutamate + 2-deoxyuridylate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
(6S)-tetrahydropteroylglutamate + 2-deoxyuridylate = 7,8-dihydropteroylglutamate + thymidine phosphate
-
methylenetetrahydropteroylglutamate + dUMP = 7,8-dihydropteroylglutamate + thymidine phosphate
-
methylenetetrahydropteroylglutamate + dUMP = 7,8-dihydropteroylglutamate + thymidine phosphate
-
methylenetetrahydropteroylglutamate + dUMP = 7,8-dihydropteroylglutamate + thymidine phosphate
-
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate = dihydrofolate + cytidine phosphate
-
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate = dihydrofolate + cytidine phosphate
-
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate = dihydrofolate + cytidine phosphate
-
5,10-methylenetetrahydrofolate + uridine 5'-phosphate = dihydrofolate + TMP
-
5,10-methylenetetrahydrofolate + uridine 5'-phosphate = dihydrofolate + TMP
-
5,10-methylenetetrahydrofolate + uridine 5'-phosphate = dihydrofolate + TMP
-
5,10-methylenetetrahydrofolate + CTP = dihydrofolate + 5-methylcytidine 5'-triphosphate
-
-
5,10-methylenetetrahydrofolate + dCTP = dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
-
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
-
10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
-
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoylglutamate = diphosphate + dihydrofolate
-
ATP + 7,8-dihydropteroate + L-Glu = ADP + phosphate + 7,8-dihydrofolate
1022, 1006, 1010, 1013, 1014, 1017, 1018, 1021, 1023, 650691, 1020, 1012, 1019, 1026, 1005, 1008, 1009, 1007, 1011, 1025, 1015, 1016, 1024, , 653172, 653665
-
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
-
-
ATP + L-glutamate + 7,8-dihydropteroate = ADP + phosphate + 7,8-dihydropteroylglutamate
-
-
Activator in Enzyme-catalyzed Reactions (1 result)
Inhibitor in Enzyme-catalyzed Reactions (47 results)
EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive with respect to 5,10-methylenetetrahydrofolate and uncompetitive with respect to NADPH
-
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme
treatment of cells with trimethoprim leads to inhibition due to accumulation of dihydrofolate through the inhibition of dihydrofolate reductase. Therefore falling dihydrofolate reductase activity leads to falling folylpolyglutamate synthase activity in a domino-like cascade
-
3D Structure of Enzyme-Ligand-Complex (PDB) (130 results)
EC NUMBER
ENZYME 3D STRUCTURE
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (204 results)
EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.05
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.49
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
1.39
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
1.7
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
KM Value (242 results)
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.00063
-
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C, value below
0.0027
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0077
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0096
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
0.014
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
Ki Value (7 results)
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0014
-
1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate
0.00178
-
5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate
References & Links
Literature References (209)
Links to other databases for 7,8-dihydrofolate
ChEBI
PubChem
-
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