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IUBMB Comments The enzyme, described from the yeast Saccharomyces cerevisiae , is involved in ribosome biogenesis.
Reaction Schemes
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uracil2634 in 25S rRNA
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N3-methyluracil2634 in 25S rRNA
Synonyms BMT5 , YIL096C,
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BMT5
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YIL096C
locus name
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S-adenosyl-L-methionine + uracil2634 in 25S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
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S-adenosyl-L-methionine:tRNA (uracil2634-N3)-methyltransferase
The enzyme, described from the yeast Saccharomyces cerevisiae, is involved in ribosome biogenesis.
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S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
Substrates: -
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S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
Substrates: -
?
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S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
Substrates: -
?
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
Substrates: -
?
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SwissProt
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SwissProt
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Highest Expressing Human Cell Lines
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malfunction
loss of bmt5 does not impair the growth of the cells at 30°C, 37°C and 19°C on YPD media. The loss of bmt5 does not exhibit any hypersensitivity to paromomycin and anisomycin. The absence of the N3-methyluracil2634 modification does not disturb ribosomal stoichiometry and affect translation
malfunction
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loss of bmt5 does not impair the growth of the cells at 30°C, 37°C and 19°C on YPD media. The loss of bmt5 does not exhibit any hypersensitivity to paromomycin and anisomycin. The absence of the N3-methyluracil2634 modification does not disturb ribosomal stoichiometry and affect translation
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BMT5_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
336
0
39402
Swiss-Prot
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His-tagged enzyme is purified by Nickel-NTA metal affinity chromatography
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plasmid pSH26 carrying N terminal 6xHis-Smt3tagged Bmt5, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37°C to an OD600 of 0.6
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Sharma, S.; Yang, J.; Duettmann, S.; Watzinger, P.; Koetter, P.; Entian, K.D.
Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae
Nucleic Acids Res.
42
3246-3260
2014
Saccharomyces cerevisiae (P40493), Saccharomyces cerevisiae ATCC 204508 (P40493)
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