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Information on EC 2.1.1.229 - tRNA (carboxymethyluridine34-5-O)-methyltransferase and Organism(s) Homo sapiens and UniProt Accession Q96BT7

for references in articles please use BRENDA:EC2.1.1.229
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IUBMB Comments
The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.
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This record set is specific for:
Homo sapiens
UNIPROT: Q96BT7
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
+
carboxymethyluridine34 in tRNA
=
+
5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
Synonyms
trna methyltransferase 9, trm9p, trm9-trm112, yml014w, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (carboxymethyluridine34-5-O)-methyltransferase
The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA
S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
show the reaction diagram
the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival
-
-
?
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ALKB8_HUMAN
664
0
75208
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the FLAG-tagged-ABH8 coding region is cloned into the pBABEpuro or pBABEhygro retroviral vector. HeLa S3 cells are infected with retrovirus
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
DNA-damaging agents induce ABH8 expression in an ATM(central DNA damage kinase)-dependent manner
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
transurethral injection of ALKBH8 small interfering RNA (siRNA) offers promise as a new therapeutic strategy for bladder cancer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shimada, K.; Nakamura, M.; Anai, S.; de Velasco, M.; Tanaka, M.; Tsujikawa, K.; Ouji, Y.; Konishi, N.
A novel human AlkB homologue, ALKBH8, contributes to human bladder cancer progression
Cancer Res.
69
3157-3164
2009
Homo sapiens (Q96BT7)
Manually annotated by BRENDA team
Fu, D.; Brophy, J.A.; Chan, C.T.; Atmore, K.A.; Begley, U.; Paules, R.S.; Dedon, P.C.; Begley, T.J.; Samson, L.D.
Human AlkB homolog ABH8 is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival
Mol. Cell. Biol.
30
2449-2459
2010
Homo sapiens (Q96BT7)
Manually annotated by BRENDA team