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Information on EC 1.7.1.6 - azobenzene reductase and Organism(s) Geobacillus stearothermophilus and UniProt Accession Q9X4K2

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EC Tree
IUBMB Comments
The reaction occurs in the reverse direction to that shown above. Other azo dyes, such as Methyl Red, Rocceline, Solar Orange and Sumifix Black B can also be reduced .
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This record set is specific for:
Geobacillus stearothermophilus
UNIPROT: Q9X4K2
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The taxonomic range for the selected organisms is: Geobacillus stearothermophilus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
azo reductase, azo-reductase, paazor1, orange ii azoreductase, azobenzene reductase, azor1, ef0404, aerobic azoreductase, orange i azoreductase, azo 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
azo dye reductase
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azo reductase
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azo-dye reductase
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azoreductase
dibromopropylaminophenylazobenzoic azoreductase
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dimethylaminobenzene reductase
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methyl red azoreductase
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N,N-dimethyl-4-phenylazoaniline azoreductase
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NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase
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NADPH2-dependent azoreductase
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NC-reductase
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new Coccine (NC)-reductase
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nicotinamide adenine dinucleotide (phosphate) azoreductase
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Orange II azoreductase
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p-aminoazobenzene reductase
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p-dimethylaminoazobenzene azoreductase
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reductase, azobenzene
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
reduction
SYSTEMATIC NAME
IUBMB Comments
N,N-dimethyl-1,4-phenylenediamine, aniline:NADP+ oxidoreductase
The reaction occurs in the reverse direction to that shown above. Other azo dyes, such as Methyl Red, Rocceline, Solar Orange and Sumifix Black B can also be reduced [2].
CAS REGISTRY NUMBER
COMMENTARY hide
9029-31-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(2-pyridylazo)-2-naphthol + NADH + H+
? + NAD+
show the reaction diagram
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ir
acid red 88 + NADH + H+
? + NAD+
show the reaction diagram
10% of hte activity with methyl red at 30°C
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ir
Congo red + NADH + H+
? + NAD+
show the reaction diagram
-
-
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ir
ethyl red + NADH + H+
? + NAD+
show the reaction diagram
best substrate, 182% of the activity with methyl red at 30°C
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ir
methyl red + 2 NADH + 2 H+
o-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
show the reaction diagram
-
-
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ir
orange I + NADH + H+
? + NAD+
show the reaction diagram
-
-
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ir
Acid Orange 52 + NADPH + H+
?
show the reaction diagram
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-
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?
Acid Red 88 + NADPH + H+
?
show the reaction diagram
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-
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-
?
Acid Yellow 23 + NADPH + H+
?
show the reaction diagram
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-
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?
Congo Red + NADPH + H+
?
show the reaction diagram
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-
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?
Direct Brown 44 + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
Direct Red 80 + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
Methyl Red + NADPH + H+
2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NADP+
show the reaction diagram
-
-
-
-
?
Mordant Orange 1 + NADPH + H+
?
show the reaction diagram
-
-
-
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?
Orange II + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
Reactive Black 5 + NADPH + H+
?
show the reaction diagram
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?
Reactive Orange 16 + NADPH + H+
?
show the reaction diagram
-
-
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-
?
Reactive Red 120 + NADPH + H+
?
show the reaction diagram
-
-
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?
Reactive Red 180 + NADPH + H+
?
show the reaction diagram
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?
Reactive Red 22 + NADPH + H+
?
show the reaction diagram
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?
Reactive Yellow 17 + NADPH + H+
?
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
NADH preferred over NADPH
NADPH
NADH is preferred over NADPH
additional information
no substrates: NADP+, NAD+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
cosubstrate NADPH, pH 7.4, 30°C
25.8
cosubstrate NADH, pH 7.4, 30°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AZOR_GEOSE
211
0
23689
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23600
2 * 23600, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 23600, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
one month, 67% residual activity
60
10 min, no loss of activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bafana, A.; Chakrabarti, T.
Lateral gene transfer in phylogeny of azoreductase enzyme
Comput. Biol. Chem.
32
191-197
2008
Bacillus sp. (in: Bacteria) (Q9FAW5), Bacillus sp. (in: Bacteria) OY1-2 (Q9FAW5), Bacillus subtilis (O07529), Bacillus subtilis 168 (O07529), Cereibacter sphaeroides (Q8GKS3), Cereibacter sphaeroides AS1.1737 (Q8GKS3), Enterococcus faecalis (Q831B2), Escherichia coli (Q8X9S9), Geobacillus stearothermophilus (Q8RR37), Pigmentiphaga kullae (Q6YAN1), Pigmentiphaga kullae K24 (Q6YAN1), Staphylococcus aureus (Q50H63), Xenophilus azovorans (Q8KU07), Xenophilus azovorans KF46F / DSM 13620 (Q8KU07)
Manually annotated by BRENDA team
Matsumoto, K.; Mukai, Y.; Ogata, D.; Shozui, F.; Nduko, J.; Taguchi, S.; Ooi, T.
Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus
Appl. Microbiol. Biotechnol.
86
1431-1438
2010
Geobacillus stearothermophilus (Q9X4K2)
Manually annotated by BRENDA team
Suzuki, H.
Remarkable diversification of bacterial azoreductases primary sequences, structures, substrates, physiological roles, and biotechnological applications
Appl. Microbiol. Biotechnol.
103
3965-3978
2019
Bacillus sp. OY1-2, Bacillus subtilis, Bacillus subtilis 168, Bacillus subtilis ATCC 6633, Bacillus subtilis ISW1214, Cereibacter sphaeroides, Cereibacter sphaeroides AS1.1737, Geobacillus stearothermophilus, Geobacillus stearothermophilus IFO 13737
Manually annotated by BRENDA team