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Information on EC 1.4.3.13 - protein-lysine 6-oxidase and Organism(s) Danio rerio and UniProt Accession F1RD85

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EC Tree
     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.13 protein-lysine 6-oxidase
IUBMB Comments
Also acts on protein 5-hydroxylysine. This enzyme catalyses the final known enzymic step required for collagen and elastin cross-linking in the biosynthesis of normal mature extracellular matrices . These reactions play an important role for the development, elasticity and extensibility of connective tissue. The enzyme is also active on free amines, such as cadaverine or benzylamine [4,5]. Some isoforms can also use [protein]-N(6)-acetyl-L-lysine as substrate deacetamidating the substrate .
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Danio rerio
UNIPROT: F1RD85
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Word Map
The taxonomic range for the selected organisms is: Danio rerio
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
lysyl oxidase, loxl2, loxl1, loxl4, loxl3, lox-pp, lysyl oxidases, lysyl oxidase-like 1, lysyl oxidase-like 2, lysyl oxidase-like, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOXL3a
A6MH32
-
lysyl oxidase-like 3a
A6MH32
isoenzyme
LOXL3b
A6MH33
-
lysyl oxidase
lysyl oxidase-like 1
isoenzyme
lysyl oxidase-like 2a
isoenzyme
lysyl oxidase-like 2b
isoenzyme
lysyl oxidase-like 3b
A6MH33
isoenzyme
lysyl oxidase-like 5a
isoenzyme
lysyl oxidase-like 5b
isoenzyme
RAS excision protein
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
oxidative deamination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
protein-L-lysine:oxygen 6-oxidoreductase (deaminating)
Also acts on protein 5-hydroxylysine. This enzyme catalyses the final known enzymic step required for collagen and elastin cross-linking in the biosynthesis of normal mature extracellular matrices [4]. These reactions play an important role for the development, elasticity and extensibility of connective tissue. The enzyme is also active on free amines, such as cadaverine or benzylamine [4,5]. Some isoforms can also use [protein]-N(6)-acetyl-L-lysine as substrate deacetamidating the substrate [6].
CAS REGISTRY NUMBER
COMMENTARY hide
99676-44-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
collagen 2a1 + O2 + H2O
?
show the reaction diagram
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
-
-
-
?
peptidyl-L-lysyl-peptide + O2 + H2O
peptidyl-allysyl-peptide + NH3 + H2O2
show the reaction diagram
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
-
-
-
?
cadaverine + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
collagen 2a1 + O2 + H2O
?
show the reaction diagram
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
-
-
-
?
peptidyl-L-lysyl-peptide + O2 + H2O
peptidyl-allysyl-peptide + NH3 + H2O2
show the reaction diagram
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
-
-
-
?
additional information
?
-
-
critical role for lysyl oxidase in zebrafish notochord morphogenesis
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
critical role for lysyl oxidase in zebrafish notochord morphogenesis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
dependent
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-aminopropionitrile
A1L1V4, A6MH29, A6MH32, A6MH33, A6MH34, A6MH35, F1QQC3, Q6NYT8
-
(1Z)-butanal thiosemicarbazone
-
-
2-mercaptopyridine-N-oxide
beta-aminopropionitrile
cyclohexanone thiosemicarbazone
-
-
cyclopentanone thiosemicarbazone
-
-
Disulfiram
-
a dithiocarbamate and Cu2+-chelator, inhibits the enzyme and causes teratogenic effects, malformations, distorted notochord development, and shortened anterior to posterior axis in zebrafish, overview
N-[2-([2-[(1-oxidopyridin-2-yl)sulfanyl]ethyl]amino)ethyl]-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide
-
fully effective at 0.02 mM
tert-butyl [2-[(1-oxidopyridin-2-yl)sulfanyl]ethyl]carbamate
-
fully effective at 0.002 mM
thiram
-
a dithiocarbamate and Cu2+-chelator, inhibits the enzyme and causes teratogenic effects, malformations, distorted notochord development, and shortened anterior to posterior axis in zebrafish, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
not expressed in the embryonic notochord
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
partial knockdown of three LOX genes, lox,loxl1 and loxl5b, sensitizes the developing embryo to dithiocarbamate exposure
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LOX3A_DANRE
739
0
82133
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
mature enzyme, SDS-PAGE
50000
-
immature enzyme, SDS-PAGE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Anderson, C.; Bartlett, S.J.; Gansner, J.M.; Wilson, D.; He, L.; Gitlin, J.D.; Kelsh, R.N.; Dowden, J.
Chemical genetics suggests a critical role for lysyl oxidase in zebrafish notochord morphogenesis
Mol. BioSyst.
3
51-59
2007
Danio rerio
Manually annotated by BRENDA team
Gansner, J.M.; Mendelsohn, B.A.; Hultman, K.A.; Johnson, S.L.; Gitlin, J.D.
Essential role of lysyl oxidases in notochord development
Dev. Biol.
307
202-213
2007
Danio rerio (A1L1V4), Danio rerio (A6MH29), Danio rerio (A6MH32), Danio rerio (A6MH33), Danio rerio (A6MH34), Danio rerio (A6MH35), Danio rerio (F1QQC3), Danio rerio (Q6NYT8), Danio rerio
Manually annotated by BRENDA team
Reynaud, C.; Baas, D.; Gleyzal, C.; Le Guellec, D.; Sommer, P.
Morpholino knockdown of lysyl oxidase impairs zebrafish development, and reflects some aspects of copper metabolism disorders
Matrix Biol.
27
547-560
2008
Danio rerio
Manually annotated by BRENDA team
van Boxtel, A.L.; Kamstra, J.H.; Fluitsma, D.M.; Legler, J.
Dithiocarbamates are teratogenic to developing zebrafish through inhibition of lysyl oxidase activity
Toxicol. Appl. Pharmacol.
244
156-161
2010
Danio rerio
Manually annotated by BRENDA team