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IUBMB Comments Also oxidizes dihydrothymine to thymine. A flavoprotein (FMN).
Synonyms
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oxidase, dihydrouracil
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5,6-dihydrouracil + O2 = uracil + H2O2
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5,6-dihydrouracil:oxygen oxidoreductase
Also oxidizes dihydrothymine to thymine. A flavoprotein (FMN).
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5,6-dihydrouracil + O2
uracil + H2O2
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Substrates: O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid
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dihydrothymine + O2
thymine + H2O2
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Substrates: -
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5-Bromouracil
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competitive vs. dihydrouracil
5-Chlorouracil
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competitive vs. dihydrouracil
5-fluorouracil
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competitive vs. dihydrouracil
5-Iodouracil
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competitive vs. dihydrouracil
5-Nitrouracil
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competitive vs. dihydrouracil
p-chloromercuribenzoate
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thymine
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competitive vs. dihydrouracil
Uracil
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competitive vs. dihydrouracil
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Highest Expressing Human Cell Lines
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6 - 10
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1 h, 25°C, stable
391073
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ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified
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Owaki, J.; Uzura, K.; Minami, Z.; Kuai, K.
Partial purification and characterization of dihydrouracil oxidase, a flavoprotein from Rhodotorula glutinis
J. Ferment. Technol.
64
205-210
1986
Rhodotorula glutinis
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