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IUBMB CommentsSome other isoflavones are reduced to the corresponding isoflavanones.
The expected taxonomic range for this enzyme is: Fusarium solani-melongenae
Synonyms
reductase, biochanin A,
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reductase, biochanin A
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dihydrobiochanin A + NADP+ = biochanin A + NADPH + H+
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dihydrobiochanin-A:NADP+ DELTA2-oxidoreductase
Some other isoflavones are reduced to the corresponding isoflavanones.
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5,7,3'-trihydroxy-4'-methoxyisoflavone + NADPH + H+
2-dihydropratensein + NADP+
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trivial name pratensein, 78% of activity with biochanin A
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5,7,3'-trihydroxyisoflavone + NADPH + H+
2-dihydrogenistein + NADP+
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trivial name genistein, 44% of activity with biochanin A
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
biochanin A + NADPH + H+
dihydrobiochanin A + NADP+
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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highly specific for 5,7-hydroxy group and some specificity for 4'-substituent of isoflavones, no reaction with flavones, chalcones, 5-deoxyisoflavones
hydrated at 2 position or ring A of isoflavone
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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trivial name biochanin A
hydrated at 2 position or ring A of isoflavone
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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trivial name biochanin A
incorporation of pro-R hydrogen from NADPH, i.e. A-specific
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
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trivial name biochanin A
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NADPH
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NADPH
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30% of maximum activity with NADH
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brenda
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brenda
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87000 - 88000
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gel filtration
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dimer
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2 * 43000-44000, SDS-PAGE
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-25°C, 20% v/v glycerol, with NADPH, but unstable with NADP+
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Schlieper, D.; Tiemann, K.; Barz, W.
Stereospecificity of hydrogen transfer by fungal and plant NADPH:isoflavone oxidoreductases
Phytochemistry
29
1519-1524
1990
Fusarium solani-melongenae
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brenda
Schlieper, D.; Barz, W.
NADPH:biochanin A oxidoreductase from the fungus Fusarium javanicum
Phytochemistry
26
2495-2498
1987
Fusarium solani-melongenae
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brenda
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1.3.1.46 biochanin-A reductase
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