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Information on EC 1.3.1.33 - protochlorophyllide reductase and Organism(s) Arabidopsis thaliana and UniProt Accession O48741

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IUBMB Comments
The enzyme catalyses a light-dependent trans-reduction of the D-ring of protochlorophyllide; the product has the (7S,8S)-configuration.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O48741
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The taxonomic range for the selected organisms is: Arabidopsis thaliana
Synonyms
nadph:protochlorophyllide oxidoreductase, protochlorophyllide oxidoreductase, nadph-protochlorophyllide oxidoreductase, por a, por b, osporb, lipor, nadph protochlorophyllide oxidoreductase, por c, light-dependent nadph:protochlorophyllide oxidoreductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Light-dependent protochlorophyllide oxidoreductase
302
-
LPOR
-
-
-
-
NADPH-Pchlide oxidoreductase
302
-
NADPH-protochlorophyllide oxidoreductase
-
-
-
-
NADPH-protochlorophyllide oxidoreductase (POR)-like protein
302
-
NADPH-protochlorophyllide oxidoreductase A
302
-
NADPH-protochlorophyllide oxidoreductase B
302
-
NADPH-protochlorophyllide reductase
-
-
-
-
NADPH2-protochlorophyllide oxidoreductase
-
-
-
-
NADPH: protochlorophyllide oxidoreductase A
302
-
NADPH:Pchlide oxidoreductase A
302
-
NADPH:protochlorophyllide (Pchlide) oxidoreductase
302
-
NADPH:protochlorophyllide oxidoreductase
302
-
NADPH:protochlorophyllide oxidoreductase A
302
-
NADPH:protochlorophyllide oxidoreductase B
302
-
protochlorophyllide oxidoreductase
protochlorophyllide oxidoreductase A
protochlorophyllide oxidoreductase B
276193
-
protochlorophyllide oxidoreductase C
protochlorophyllide photooxidoreductase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
chlorophyllide-a:NADP+ 7,8-oxidoreductase
The enzyme catalyses a light-dependent trans-reduction of the D-ring of protochlorophyllide; the product has the (7S,8S)-configuration.
CAS REGISTRY NUMBER
COMMENTARY hide
68518-04-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
protochlorophyllide + NADPH + H+
chlorophyllide + NADP+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADH + H+
chlorophyllide a + NAD+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADPH
chlorophyllide + NADP+
show the reaction diagram
protochlorophyllide + NADPH + H+
chlorophyllide + NADP+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADPH + H+
chlorophyllide a + NADP+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protochlorophyllide + NADPH + H+
chlorophyllide + NADP+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADH + H+
chlorophyllide a + NAD+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADPH
chlorophyllide + NADP+
show the reaction diagram
protochlorophyllide + NADPH + H+
chlorophyllide + NADP+
show the reaction diagram
-
-
-
-
?
protochlorophyllide + NADPH + H+
chlorophyllide a + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
phosphatidylglycerol and sulfoquinovosyl diacylglycerol increase the affinity towards NADPH a 100fold
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0268 - 0.0595
NADPH
0.00015 - 0.00039
protochlorophyllide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
activity assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
undetectable in dark-grown cells, light response during greening. Expression in mature cells
Manually annotated by BRENDA team
-
translocon constituent toc33 is indispensable for the import of isoform PorB
Manually annotated by BRENDA team
-
high expression in dark-grown cells, no light response during greening. Constitutive enzym, expression in mature cells; high expression in dark-grown cells, no light response during greening. Very low expression in mature cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
PORC_ARATH
401
0
43883
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
x * 37000, SDS-PAGE
40150
-
x * 40150, recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
POR proteins in Arabidopsis consist of dimers
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C276A
-
the mutant is hypersensitive to high-light conditions during greening
Cys303A
-
the mutant is hypersensitive to high-light conditions during greening
R321A
-
inactive
T316A/T317A
-
inactive
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the chaperone-like protein of por1 (CPP1) plays a role in the regulation of enzyme stability and function
-
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
using a chitin bead column, the chitin-intein tag is removed
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
cDNAs and/or gens encoding POR, also referred to as LPCR or PCR in some studies isolated; full-length cDNA, coding for PCR
-
expressed in Escherichia coli strain SG13009
-
expression in Escherichia coli BL21
-
full-length cDNA, coding for PCR
-
full-length cDNA, coding for PCR; POR C cloned and expressed in Escherichia coli
-
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells
-
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells, for the construction of transgenic plants pDONR/ZeO and pGWB2 vectors are used
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schulz, R.; Steinmueller, K.; Klaas, M.; Forreiter, C.; Rasmussen, S.; Hiller, C.; Apel, K.
Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli
Mol. Gen. Genet.
217
355-361
1989
Arabidopsis thaliana, Hordeum vulgare, Triticum aestivum
Manually annotated by BRENDA team
Armstrong, G.A.; Runge, S.; Frick, G.; Sperling, U.; Apel, K.
Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana
Plant Physiol.
108
1505-1517
1995
Arabidopsis thaliana, Avena sativa, Hordeum vulgare, Lepidium sativum, Pinus mugo, Pinus strobus, Pinus taeda, Pisum sativum, Triticum aestivum, Zea mays
Manually annotated by BRENDA team
Chahdi, M.A.O.; Schoefs, B.; Franck, F.
Isolation and characterization of photoactive complexes of NADPH:protochlorophyllide oxidoreductase from wheat
Planta
206
673-680
1998
Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Phaseolus vulgaris, Pisum sativum, Triticum aestivum
-
Manually annotated by BRENDA team
Klement, H.; Helfrich, M.; Oster, U.; Schoch, S.; Rudiger, W.
Pigment-free NADPH:protochlorophyllide oxidoreductase from Avena sativa L: purification and substrate specificity
Eur. J. Biochem.
265
862-874
1999
Arabidopsis thaliana, Avena sativa, Hordeum vulgare, Pisum sativum
Manually annotated by BRENDA team
Lebedev, N.; Timko, M.P.
Protochlorophyllide oxidoreductase B-catalyzed protochlorophyllide photoreduction in vitro: insight into the mechanism of chlorophyll formation in light-adapted plants
Proc. Natl. Acad. Sci. USA
96
9954-9959
1999
Arabidopsis thaliana, Hordeum vulgare
Manually annotated by BRENDA team
Franck, F.; Sperling, U.; Frick, G.; Pochert, B.; Van Cleve, B.; Apel, K.; Armstrong, G.A.
Regulation of etioplast pigment-protein complexes, inner membrane architecture, and protochlorophyllide a chemical heterogeneity by light-dependent NADPH:protochlorophyllide oxidoreductases A and B
Plant Physiol.
124
1678-1696
2000
Arabidopsis thaliana, Avena sativa
Manually annotated by BRENDA team
Su, Q.; Frick, G.; Armstrong, G.; Apel, K.
POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light
Plant Mol. Biol.
47
805-813
2001
Arabidopsis thaliana, Chlamydomonas reinhardtii, Cucumis sativus, Cucurbita pepo, Hordeum vulgare, Leptolyngbya boryana, Marchantia paleacea, Pinus mugo, Pinus strobus, Pinus taeda, Pisum sativum, Rhodobacter capsulatus
Manually annotated by BRENDA team
Pattanayak, G.K.; Tripathy, B.C.
Catalytic function of a novel protein protochlorophyllide oxidoreductase C of Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
291
921-924
2002
Arabidopsis thaliana, Arabidopsis thaliana (O48741)
Manually annotated by BRENDA team
Masuda, T.; Takamiya, K.i.
Novel insights into the enzymology, regulation and physiological functions of light-dependent protochlorophyllide oxidoreductase in angiosperms
Photosyn. Res.
81
1-29
2004
Amaranthus tricolor, Anabaena sp., Arabidopsis thaliana (O48741), Arabidopsis thaliana (P21218), Arabidopsis thaliana (Q42536), Avena sativa (P15904), Bigelowiella natans, Chlamydomonas reinhardtii (Q39617), Cucumis sativus (Q41249), Daucus carota (Q9SDT1), Gloeobacter violaceus, Hordeum vulgare (P13653), Hordeum vulgare (Q42850), Lactuca sativa, Leptolyngbya boryana, Marchantia paleacea (O80333), Nicotiana tabacum (Q8LSZ2), Nicotiana tabacum (Q8LSZ3), Oryza sativa, Pinus mugo, Pinus mugo (Q41203), Pinus strobus, Pinus taeda, Pisum sativum, Prochlorococcus marinus, Solanum lycopersicum, Synechocystis sp. (Q59987), Thermosynechococcus elongatus BP-1, Triticum aestivum (Q41578), Vigna radiata (Q9LKH8)
Manually annotated by BRENDA team
Masuda, T.; Fusada, N.; Oosawa, N.; Takamatsu, K.; Yamamoto, Y.Y.; Ohto, M.; Nakamura, K.; Goto, K.; Shibata, D.; Shirano, Y.; Hayashi, H.; Kato, T.; Tabata, S.; Shimada, H.; Ohta, H.; Takamiya, K.
Functional analysis of isoforms of NADPH: protochlorophyllide oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana
Plant Cell Physiol.
44
963-974
2003
Arabidopsis thaliana
Manually annotated by BRENDA team
Reinbothe, S.; Pollmann, S.; Springer, A.; James, R.J.; Tichtinsky, G.; Reinbothe, C.
A role of Toc33 in the protochlorophyllide-dependent plastid import pathway of NADPH:protochlorophyllide oxidoreductase (POR) A
Plant J.
42
1-12
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Kim, C.; Ham, H.; Apel, K.
Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings
Plant J.
42
329-340
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Pollmann, S.; Springer, A.; Buhr, F.; Lahroussi, A.; Samol, I.; Bonneville, J.M.; Tichtinsky, G.; von Wettstein, D.; Reinbothe, C.; Reinbothe, S.
A plant porphyria related to defects in plastid import of protochlorophyllide oxidoreductase A
Proc. Natl. Acad. Sci. USA
104
2019-2023
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Schemenewitz, A.; Pollmann, S.; Reinbothe, C.; Reinbothe, S.
A substrate-independent, 14:3:3 protein-mediated plastid import pathway of NADPH:protochlorophyllide oxidoreductase A
Proc. Natl. Acad. Sci. USA
104
8538-8543
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Masuda, S.; Ikeda, R.; Masuda, T.; Hashimoto, H.; Tsuchiya, T.; Kojima, H.; Nomata, J.; Fujita, Y.; Mimuro, M.; Ohta, H.; Takamiya, K.
Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis
Plant J.
58
952-960
2009
Arabidopsis thaliana (O48741), Arabidopsis thaliana (P21218), Arabidopsis thaliana (Q42536), Gloeobacter violaceus, Synechocystis sp. PCC 6803
Manually annotated by BRENDA team
Yuan, M.; Zhang, D.W.; Zhang, Z.W.; Chen, Y.E.; Yuan, S.; Guo, Y.R.; Lin, H.H.
Assembly of NADPH: protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings
J. Plant Physiol.
169
1311-1316
2012
Arabidopsis thaliana, Hordeum vulgare
Manually annotated by BRENDA team
Paddock, T.; Lima, D.; Mason, M.E.; Apel, K.; Armstrong, G.A.
Arabidopsis light-dependent protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development
Plant Mol. Biol.
78
447-460
2012
Arabidopsis thaliana
Manually annotated by BRENDA team
Kim, C.; Apel, K.
Arabidopsis light-dependent NADPH: protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development: an addendum
Plant Mol. Biol.
80
237-240
2012
Arabidopsis thaliana
Manually annotated by BRENDA team
Pattanayak, G.; Tripathy, B.
Overexpression of protochlorophyllide oxidoreductase c regulates oxidative stress in arabidopsis
PLoS ONE
6
e26532
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Gabruk, M.; Mysliwa-Kurdziel, B.; Kruk, J.
MGDG, PG and SQDG regulate the activity of light-dependent protochlorophyllide oxidoreductase
Biochem. J.
474
1307-1320
2017
Arabidopsis thaliana
Manually annotated by BRENDA team
Gabruk, M.; Mysliwa-Kurdziel, B.
Light-dependent protochlorophyllide oxidoreductase: phylogeny, regulation, and catalytic properties
Biochemistry
54
5255-5262
2015
Arabidopsis thaliana
Manually annotated by BRENDA team
Lee, J.Y.; Lee, H.S.; Song, J.Y.; Jung, Y.J.; Reinbothe, S.; Park, Y.I.; Lee, S.Y.; Pai, H.S.
Cell growth defect factor1/chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis
Plant Cell
25
3944-3960
2013
Arabidopsis thaliana, Nicotiana benthamiana
Manually annotated by BRENDA team
Buhr, F.; Lahroussi, A.; Springer, A.; Rustgi, S.; von Wettstein, D.; Reinbothe, C.; Reinbothe, S.
NADPH:protochlorophyllide oxidoreductase B (PORB) action in Arabidopsis thaliana revisited through transgenic expression of engineered barley PORB mutant proteins
Plant Mol. Biol.
94
45-59
2017
Arabidopsis thaliana
Manually annotated by BRENDA team
Reinbothe, S.; Gray, J.; Rustgi, S.; von Wettstein, D.; Reinbothe, C.
Cell growth defect factor 1 is crucial for the plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
112
5838-5843
2015
Arabidopsis thaliana
Manually annotated by BRENDA team
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