EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.33 | C103S |
the BchN subunit variant shows 0.5% residual activity and is essentially inactive |
687688 |
| 1.3.1.33 | C104A |
no detectable effect on the import of protein to plastid and processing in darkness |
676012 |
| 1.3.1.33 | C166A |
no detectable effect on the import of protein to plastid and processing in darkness |
676012 |
| 1.3.1.33 | C195A |
mutant, constructed for the identification of the protochlorophyllide binding site |
700943 |
| 1.3.1.33 | C199/C226S |
mutant, mutation of the absolutely conserved cysteine residues |
699004 |
| 1.3.1.33 | C199S |
kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) comparable to wild-type, Kd (protochlorophyllide) comparable to wild-type |
699004 |
| 1.3.1.33 | C199S |
mutant, Cys199 has a relatively minor role in catalysis |
699004 |
| 1.3.1.33 | C199S/C226S |
kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 2fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type |
699004 |
| 1.3.1.33 | C21S |
the BchN subunit variant shows 0.5% residual activity and is essentially inactive |
687688 |
| 1.3.1.33 | C222A |
mutant, constructed for the identification of the protochlorophyllide binding site |
700943 |
