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Information on EC 1.2.1.96 - 4-hydroxybenzaldehyde dehydrogenase (NADP+) and Organism(s) Pseudomonas putida and UniProt Accession Q59702

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EC Tree
IUBMB Comments
Involved in the aerobic pathway for degradation of toluene, 4-methylphenol, and 2,4-xylenol by several Pseudomonas strains. The enzyme is also active with 4-hydroxy-3-methylbenzaldehyde. cf. EC 1.2.1.64, 4-hydroxybenzaldehyde dehydrogenase (NAD+).
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This record set is specific for:
Pseudomonas putida
UNIPROT: Q59702
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The taxonomic range for the selected organisms is: Pseudomonas putida
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
phbdd, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
p-hydroxybenzaldehyde dehydrogenase
-
p-hydroxybenzaldehyde dehydrogenase
-
-
PchA
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenation
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzaldehyde:NADP+ oxidoreductase
Involved in the aerobic pathway for degradation of toluene, 4-methylphenol, and 2,4-xylenol by several Pseudomonas strains. The enzyme is also active with 4-hydroxy-3-methylbenzaldehyde. cf. EC 1.2.1.64, 4-hydroxybenzaldehyde dehydrogenase (NAD+).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-hydroxy-3-methylbenzaldehyde + NAD+ + H2O
4-hydroxy-3-methylbenzoic acid + NADH + H+
show the reaction diagram
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxy-3-methylbenzaldehyde + NADP+ + H2O
4-hydroxy-3-methylbenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoic acid + NADH + H+
show the reaction diagram
low activity with NAD+ as cosubstrate
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
show the reaction diagram
-
involved in the degradation pathway of p-cresol
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoic acid + NADPH + H+
show the reaction diagram
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADP+ + H2O
4-hydroxybenzoate + NADPH + H+
show the reaction diagram
-
involved in the degradation pathway of p-cresol
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
dependent on
NAD+
-
dependent on
NADP+
-
4fold greater oxidation than with NAD+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00636 - 0.02055
4-hydroxy-3-methylbenzaldehyde
0.00357 - 0.01311
4-hydroxybenzaldehyde
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017 - 0.071
4-hydroxy-3-methylbenzaldehyde
0.0017 - 0.26
4-hydroxybenzaldehyde
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.083 - 11.16
4-hydroxy-3-methylbenzaldehyde
0.13 - 72.83
4-hydroxybenzaldehyde
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PCHA_PSEPU
491
0
53156
Swiss-Prot
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cronin, C.N.; Kim, J.; Fuller, J.H.; Zhang, X.; McIntire, W.S.
Organization and sequences of p-hydroxybenzaldehyde dehydrogenase and other plasmid-encoded genes for early enzymes of the p-cresol degradative pathway in Pseudomonas putida NCIMB 9866 and 9869
DNA Seq.
10
7-17
1999
Pseudomonas putida
Manually annotated by BRENDA team
Chen, Y.F.; Chao, H.; Zhou, N.Y.
The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866
Appl. Microbiol. Biotechnol.
98
1349-1356
2014
Pseudomonas putida (Q59702), Pseudomonas putida NCIMB 9866 (Q59702)
-
Manually annotated by BRENDA team
Miller Jr, E.S.; Peretti, S.W.
Bioconversion of toluene to p-hydroxybenzoate via the construction and characterization of a recombinant Pseudomonas putida
Green Chem.
1
143-152
1999
Pseudomonas putida, Pseudomonas putida EM2839
-
Manually annotated by BRENDA team