BRENDA - Enzyme Database
show all sequences of 1.2.1.96

Bioconversion of toluene to p-hydroxybenzoate via the construction and characterization of a recombinant Pseudomonas putida

Miller Jr, E.S.; Peretti, S.W.; Green Chem. 1, 143-152 (1999)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
Pseudomonas putida
-
4-hydroxybenzoate + NADH + H+
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
Pseudomonas putida EM2839
-
4-hydroxybenzoate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida EM2839
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
-
733948
Pseudomonas putida
4-hydroxybenzoate + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
733948
Pseudomonas putida EM2839
4-hydroxybenzoate + NADH + H+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
Pseudomonas putida
-
4-hydroxybenzoate + NADH + H+
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
Pseudomonas putida EM2839
-
4-hydroxybenzoate + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybenzaldehyde + NAD+ + H2O
-
733948
Pseudomonas putida
4-hydroxybenzoate + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
-
733948
Pseudomonas putida EM2839
4-hydroxybenzoate + NADH + H+
-
-
-
?
Other publictions for EC 1.2.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741220
Mukherjee
Redirection of metabolite bios ...
Daucus carota
Planta
243
305-320
2016
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726788
Chen
-
The catabolism of 2,4-xylenol ...
Pseudomonas putida, Pseudomonas putida NCIMB 9866
Appl. Microbiol. Biotechnol.
98
1349-1356
2014
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1
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4
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2
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1
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8
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1
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4
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4
4
288312
Cronin
Organization and sequences of ...
Pseudomonas putida
DNA Seq.
10
7-17
1999
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1
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733948
Miller Jr
-
Bioconversion of toluene to p- ...
Pseudomonas putida, Pseudomonas putida EM2839
Green Chem.
1
143-152
1999
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-
-
-
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2
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2
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2
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1
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1
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2
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2
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288314
Whited
Separation and partial charact ...
Pseudomonas mendocina, Pseudomonas mendocina KR1
J. Bacteriol.
173
3017-3020
1991
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2
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2
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1
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1
1
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2
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1
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2
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1
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