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Information on EC 1.2.1.13 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) and Organism(s) Bacillus subtilis and UniProt Accession O34425

for references in articles please use BRENDA:EC1.2.1.13
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Bacillus subtilis
UNIPROT: O34425 not found.
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
glyceraldehyde-3-p dehydrogenase, nadp-glyceraldehyde-3-phosphate dehydrogenase, gapa1, nadp-gapdh, gapa-1, glyceraldehyde-3-dehydrogenase, nadp-gpd, photosynthetic gapdh, ov-gapdh, glyceraldehyde-p dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADP+-dependent gapB
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dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating)
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GAPDH
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glyceraldehyde 3-phosphate dehydrogenase (NADP)
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glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating)
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glyceraldehyde-P dehydrogenase
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NAD(P)-GAPDH
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NADP+-dependent G-3-P dehydrogenase
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NADP-dependent glyceraldehyde phosphate dehydrogenase
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NADP-dependent glyceraldehydephosphate dehydrogenase
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NADP-glyceraldehyde phosphate dehydrogenase
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NADP-glyceraldehyde-3-phosphate dehydrogenase
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NADP-GPD
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NADP-triose phosphate dehydrogenase
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NADPH-D-GA3P
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triosephosphate dehydrogenase (NADP+)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate:NADP+ oxidoreductase (phosphorylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-87-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate + phosphate + NADP+
3-phospho-D-glyceroyl phosphate + NADPH + H+
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
to increase NADPH bioavailability, the native NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gapA gene, EC 1.2.1.12, in Escherichia coli is replaced with the NADP+-dependent gapB gene, EC 1.2.1.13, from Bacillus subtilis. To overcome the limitation of NADP+ availability, Escherichia coli NAD kinase, gene nadK is also coexpressed with gapB in Escherichia coli. replacing NAD+-dependent GapA activity with NADP+-dependent GapB activity increases the synthesis of NADPH-dependent compounds
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene gapB, recombinant overexpression in and complementation of Escherichia coli gapA-deficient strain MG1655 gapA::Tn10, gene gapB is expressed from plasmid pDHC29 under the Plac promoter control, coexpression with Escherichia coli gene nadK encoding the NAD kinase, genes gapB and nadk are grouped as a single operon
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wang, Y.; San, K.Y.; Bennett, G.N.
Improvement of NADPH bioavailability in Escherichia coli by replacing NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase GapA with NADP+-dependent GapB from Bacillus subtilis and addition of NAD kinase
J. Ind. Microbiol. Biotechnol.
40
1449-1460
2013
Bacillus subtilis (O34425), Bacillus subtilis 168 (O34425)
Manually annotated by BRENDA team