Information on EC 1.14.99.47 - (+)-larreatricin hydroxylase

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The expected taxonomic range for this enzyme is: Larrea tridentata

EC NUMBER
COMMENTARY hide
1.14.99.47
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RECOMMENDED NAME
GeneOntology No.
(+)-larreatricin hydroxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-larreatricin + AH2 + O2 = (+)-3'-hydroxylarreatricin + A + H2O
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
(+)-larreatricin:oxygen 3'-hydroxylase
Isolated from the plant Larrea tridentata (creosote bush). The enzyme has a strong preference for the 3' position of (+)-larreatricin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-larreatricin + O2 + AH2
(+)-3-hydroxylarreatricin + A + H2O
show the reaction diagram
-
enzyme cataylzes the enantio-specific conversion of (+)-larreatricin into (+)-3-hydroxylarreatricin, with the regiochemistry of catalysis being unambiguously established. The corresponding (-)-enantiomer does not serve as a substrate
-
?
3'-hydroxy-larreatricin + O2
3,3'-dihydroxylarreatricin
show the reaction diagram
-
3-hydroxylation
-
-
?
3-hydroxy-larreatricin + O2
3,3'-dihydroxylarreatricin
show the reaction diagram
-
3'-hydroxylation
-
-
?
larreatricin + O2
3'-hydroxy-larreatricin
show the reaction diagram
-
3'-hydroxylation
-
-
?
larreatricin + O2
3-hydroxy-larreatricin
show the reaction diagram
-
3-hydroxylation
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-hydroxy-larreatricin + O2
3,3'-dihydroxylarreatricin
show the reaction diagram
-
3-hydroxylation
-
-
?
3-hydroxy-larreatricin + O2
3,3'-dihydroxylarreatricin
show the reaction diagram
-
3'-hydroxylation
-
-
?
larreatricin + O2
3'-hydroxy-larreatricin
show the reaction diagram
-
3'-hydroxylation
-
-
?
larreatricin + O2
3-hydroxy-larreatricin
show the reaction diagram
-
3-hydroxylation
-
-
?
additional information
?
-
-
the Larrea tridentate PPO gene product acts as a (+)-larreatricin 3'-hydroxylase in vivo
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
a copper-containing enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.2
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pH 7.0, 30°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
Larrea tridentata PPO contains N-terminal sequences predicting its localization to the chloroplast thylakoid lumen
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
x * 43000, SDS-PAGE, x * 66352, calculated
66352
-
x * 43000, SDS-PAGE, x * 66352, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 43000, SDS-PAGE, x * 66352, calculated
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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enzyme is subject to posttranslational processing by removal of an 8.7-kDa N-terminal transit peptide, residues 1-79, and an 17-kDa COOH-terminal peptide, residues 432–584
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene PPO, DNA and amino acid sequence determination and analyis
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