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The expected taxonomic range for this enzyme is: Pseudomonas sp.
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3-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD+
4-bromophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Br- + NAD+
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
4-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
4-fluorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + F- + NAD+
4-hydroxyphenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + OH- + NAD+
-
4% of the activity of 4-chlorophenylacetate
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-
?
phenylacetate + NADH + O2 + H2O
?
-
6% of the activity of 4-chlorophenylacetate
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-
?
additional information
?
-
3-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
10% of the activity of 4-chlorophenylacetate
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-
?
3-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD+
-
10% of the activity of 4-chlorophenylacetate
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Br- + NAD+
-
102% of the activity of 4-chlorophenylacetate
-
-
?
4-bromophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Br- + NAD+
-
102% of the activity of 4-chlorophenylacetate
-
-
?
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
-
r
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
-
r
4-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
-
?
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
-
r
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
-
r
4-fluorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + F- + NAD+
-
30% of the activity of 4-chlorophenylacetate
-
-
?
4-fluorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + F- + NAD+
-
30% of the activity of 4-chlorophenylacetate
-
-
?
additional information
?
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the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
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-
?
additional information
?
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not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
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-
?
additional information
?
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not active as substrate: benzoate, substituted analogues of benzoate, 2-chlorophenylacetate, antipyrin, 4-chlorocinnamate
-
-
?
additional information
?
-
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the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
-
-
?
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monomer
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1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
monomer
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1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
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trimer
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3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
trimer
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3 * 46000-52000, dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
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additional information
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the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
additional information
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the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
additional information
-
the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
-
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Markus, A.; Krekel, D.; Lingens, F.
Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS
J. Biol. Chem.
261
12883-12888
1986
Pseudomonas sp., Pseudomonas sp. CBS 3
brenda
Schweizer, D.; Markus, A.; Seez, M.; Ruf, H.H.; Lingens, F.
Purification and some properties of component B of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS 3
J. Biol. Chem.
262
9340-9346
1987
Pseudomonas sp., Pseudomonas sp. CBS 3
brenda
1.14.12.9 4-chlorophenylacetate 3,4-dioxygenase
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