Information on EC 1.14.12.1 - anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

for references in articles please use BRENDA:EC1.14.12.1
Word Map on EC 1.14.12.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

top print hide
EC NUMBER
COMMENTARY hide
1.14.12.1
-
top print hide
RECOMMENDED NAME
GeneOntology No.
anthranilate 1,2-dioxygenase (deaminating, decarboxylating)
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
anthranilate + NAD(P)H + 2 H+ + O2 = catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
-
-
-
-
decarboxylation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-nitrobenzoate degradation II
-
-
anthranilate degradation I (aerobic)
-
-
indole-3-acetate degradation
-
-
Aminobenzoate degradation
-
-
Microbial metabolism in diverse environments
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating)
Requires Fe2+.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
9059-17-0
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
wild-type ATCC 15926, ATCC 29574
-
-
Manually annotated by BRENDA team
No.23 (ATCC 11250)
-
-
Manually annotated by BRENDA team
two component type of enzyme
-
-
Manually annotated by BRENDA team
two component type of enzyme
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
anthranilate + NADPH + H+ + O2
catechol + CO2 + NADP+ + NH3
show the reaction diagram
-
-
-
-
?
anthranilic acid + alanine + O2
?
show the reaction diagram
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
show the reaction diagram
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
anthranilic acid + NADH + O2 + H2O
catechol + CO2 + NAD+ + NH3
show the reaction diagram
-
-
-
-
?
benzoate + NAD(P)H + O2 + H2O
benzoate dihydrodiol + ?
show the reaction diagram
catechol + O2 + NAD(P)H + H2O
?
show the reaction diagram
-
-
-
-
?
protocatechuic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
salicylic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
-
salicylic acid and o-aminophenol are no substrates
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
anthranilate + NADPH + H+ + O2
catechol + CO2 + NADP+ + NH3
show the reaction diagram
-
-
-
-
?
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
show the reaction diagram
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-sulfur centre
NADPH
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
required
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
-
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
oxygenase component completely inactive at pH 9
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18900
AndAd, product of ORF4, calculated from deduced amino acid sequence; AndAd, product of ORF4, calculated from deduced amino acid sequence
19000
-
1 * 19000 + 1 * 39000 + 1 * 54000, DNA-sequence
24000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
39000
-
1 * 19000 + 1 * 39000 + 1 * 54000, DNA-sequence
48000
AndAc, product of ORF3, calculated from deduced amino acid sequence; AndAc, product of ORF3, calculated from deduced amino acid sequence
52000
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
54000
-
1 * 19000 + 1 * 39000 + 1 * 54000, DNA-sequence
220000
-
gel filtration, wild type and mutant enzymes
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
trimer
-
1 * 19000 + 1 * 39000 + 1 * 54000, DNA-sequence
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein, expressed in Escherichia coli
-
wild type and mutant enzymes
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
clustered antABC genes from ADP1 chromosome encodes anthranilate dioxygenase
-
clustered antABC genes from ADP1 chromosome encodes anthranilate dioxygenase; expressed in Escherichia coli
-
expressed in Escherichia coli TOP10F'; expressed in Escherichia coli TOP10F'
expressed in Escherichia coli W3110 trpD9923 cells
-
expression of mutant enzymes in Escherichia coli BL21-Codon Plus
-
top print hide
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D218A
-
no activity
D218E
-
no activity
D218N
-
no activity
top
Show AA Sequence (540 entries)
Please use the Sequence Search for a specific query.
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.14.12.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)