Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.11.1.7 - peroxidase and Organism(s) Gossypium hirsutum and UniProt Accession Q8RVP6

for references in articles please use BRENDA:EC1.11.1.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.7 peroxidase
IUBMB Comments
Heme proteins with histidine as proximal ligand. The iron in the resting enzyme is Fe(III). They also peroxidize non-phenolic substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Certain peroxidases (e.g. lactoperoxidase, SBP) oxidize bromide, iodide and thiocyanate.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Gossypium hirsutum
UNIPROT: Q8RVP6
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Gossypium hirsutum
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
2
phenolic donor
+
=
2
phenoxyl radical of the donor
+
2
Synonyms
horseradish peroxidase, horseradish peroxidase (hrp), rhepo, lactoperoxidase, eosinophil peroxidase, guaiacol peroxidase, heme peroxidase, rubrerythrin, cyp119, thiol peroxidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cPOD-I
-
isozyme
eosinophil peroxidase
-
-
-
-
extensin peroxidase
-
-
-
-
guaiacol peroxidase
-
-
-
-
heme peroxidase
-
-
-
-
horseradish peroxidase (HRP)
-
-
-
-
Japanese radish peroxidase
-
-
-
-
lactoperoxidase
-
-
-
-
MPO
-
-
-
-
myeloperoxidase
-
-
-
-
oxyperoxidase
-
-
-
-
protoheme peroxidase
-
-
-
-
pyrocatechol peroxidase
-
-
-
-
rPOD-II
scopoletin peroxidase
-
-
-
-
thiocyanate peroxidase
-
-
-
-
verdoperoxidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
phenolic donor:hydrogen-peroxide oxidoreductase
Heme proteins with histidine as proximal ligand. The iron in the resting enzyme is Fe(III). They also peroxidize non-phenolic substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Certain peroxidases (e.g. lactoperoxidase, SBP) oxidize bromide, iodide and thiocyanate.
CAS REGISTRY NUMBER
COMMENTARY hide
9003-99-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylcatechol + H2O2
?
show the reaction diagram
28% activity compared to guaiacol
-
-
?
benzoic acid + H2O2
?
show the reaction diagram
10% activity compared to guaiacol
-
-
?
catechin + H2O2
?
show the reaction diagram
19% activity compared to guaiacol
-
-
?
catechol + H2O2
?
show the reaction diagram
36% activity compared to guaiacol
-
-
?
chlorogenic acid + H2O2
?
show the reaction diagram
80% activity compared to guaiacol
-
-
?
guaiacol + H2O2
tetraguaiacol + H2O
show the reaction diagram
100% activity with guaiacol at 25 mM
-
-
?
p-coumaric acid + H2O2
?
show the reaction diagram
13% activity compared to guaiacol
-
-
?
pyrogallol + H2O2
?
show the reaction diagram
90% activity compared to guaiacol
-
-
?
tetramethylbenzidine + H2O2
?
show the reaction diagram
215% activity compared to guaiacol
-
-
?
4-methylcatechol + H2O2
?
show the reaction diagram
benzoic acid + H2O2
?
show the reaction diagram
catechin + H2O2
?
show the reaction diagram
catechol + H2O2
?
show the reaction diagram
chlorogenic acid + H2O2
?
show the reaction diagram
guaiacol + H2O2
tetraguaiacol + H2O
show the reaction diagram
p-coumaric acid + H2O2
?
show the reaction diagram
pyrogallol + H2O2
?
show the reaction diagram
84% activity compared to guaiacol
-
-
?
pyrogallol + H2O2
purpurogallin + H2O
show the reaction diagram
tetramethylbenzidine + H2O2
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Al3+
77% increased activity at 1 mM
Ca2+
40% increased activity at 1 mM
Fe3+
193% increased activity at 1 mM
Ni2+
9% increased activity at 1 mM
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbic acid
complete inhibition at 1 mM
beta-mercaptoethanol
96% inhibition at 1 mM
cysteine
73% inhibition at 1 mM
dithiothreitol
complete inhibition at 1 mM
EDTA
28% inhibition at 1 mM
Hg2+
59% inhibition at 1 mM
K+
27% inhibition at 1 mM
KI
36% inhibition at 1 mM
Mn2+
30% inhibition at 1 mM
Na+
44% inhibition at 1 mM
Sodium metabisulfite
complete inhibition at 1 mM
Tween 80
45% inhibition at 1 mM
Zn2+
62% inhibition at 1 mM
ascorbic acid
complete inhibition at 1 mM
beta-mercaptoethanol
cysteine
dithiothreitol
Na+
49% inhibition at 1 mM
NaCl
44% inhibition at 1 mM
Sodium metabisulfite
complete inhibition at 1 mM
Tween 80
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivar Coker 312
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8RVP6_GOSHI
329
0
36492
TrEMBL
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
the enzyme retains more than 90% of its initial activity within the pH range 5.0-6.0, weakest activity at pH 8.0, enzymatic activity is lowest after incubation for 60 min at pH 7.0 and 8.0
695687
4 - 8
the enzyme retains more than 90% of its initial activity within the pH range 6.0-7.0, weakest activity at pH 8.0, enzymatic activity is lowest after incubation for 60 min at pH 4.0 and 8.0
695687
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 80
relatively stable between 20-30°C, activity decreases by 25% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min
20 - 70
relatively stable at 20-30°C, a decrease in POD activity of 75%, 85%, and 90% is found after 10 min at 50°C, 60°C, and 70°C, respectively. The enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min, respectively.
20 - 80
relatively stable between 20-30°C, activity decreases by 20% at 40°C, the time required for 50% inactivation of activity at 50°C, 60°C, and 70°C is about 5 min, a decrease in POD activity of 75%, 85%, and 90% occurs after 10 min at 50°C, 60°C, and 70°C, respectively, the enzyme activity at 50°C, 60°C, and 70-80°C is completely exhausted in 40, 25, and 10 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Sephadex G-200 gel filtration
ammonium sulfate precipitation and Sephadex G-200 gel filtration
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kouakou, T.H.; Due, E.A.; Kouadio, N.E.; Niamke, S.; Kouadio, Y.J.; Merillon, J.M.
Purification and characterization of cell suspensions peroxidase from cotton (Gossypium hirsutum L.)
Appl. Biochem. Biotechnol.
157
575-592
2008
Gossypium hirsutum, Gossypium hirsutum (Q8RVP6), Gossypium hirsutum (Q8RVW0)
Manually annotated by BRENDA team