EC Number   |
Cofactor |
Reference |
|---|
    1.11.1.7 | heme |
- |
439739, 439747, 439751, 439752, 439754, 671338, 672519, 674928, 688396, 695576, 695687, 696298, 696482, 696500, 696779, 697530, 698044, 698048, 698420, 698897, 698919, 699011, 699269, 700707, 701107, 710744, 711160, 711361, 711550, 712224, 712226, 712714, 713470, 713517, 724110, 724400, 724412, 725055, 725088, 725143, 725507, 726432 |
| 1.11.1.7 | heme |
1.3 mol heme per molecule, protoheme |
439741 |
| 1.11.1.7 | heme |
contains 2 mol of heme per mol of the enzyme. Iron of heme is in Fe-III state |
676949 |
| 1.11.1.7 | heme |
enzyme contains a high-spin heme. The FeIII/FeII reduction potential is -266 mV vs normal hydrogen electrode. The gamma-heme edge shows a substrate binding site |
742917 |
| 1.11.1.7 | heme |
holo-form binds two c-type hemes covalently bound to the polypeptide chain, a high-potential E heme and a low-potential P heme, with redox potentials of (+310 mV) and (-190 mV/-300 mV), respectively in the presence of calcium ions, at pH 7.5. A calcium dependent reductive activation mechanism is present, in which P heme is bis-His coordinated low-spin in the fully oxidized state of the enzyme, and becomes penta-coordinated high-spin upon reduction of E heme in the presence of calcium ions. The rate-limiting step in the catalytic cycle is the electron transfer between the two hemes |
743044 |
| 1.11.1.7 | heme |
iron protoporphyrin-containing heme peroxidase |
695785 |
| 1.11.1.7 | heme |
isozyme VPO1 has a heme content of 1.64 nmol/mg protein |
698048 |
| 1.11.1.7 | heme |
loss of a structural restraint in the proximal heme pocket of mutant enzyme T171S allows slippage of the proximal heme ligand, but only in the reduced state. This is a remarkably subtle and specific effect that appears to increase the flexibility of the reduced state of the mutant compared to that of the wild-type protein |
673533 |
| 1.11.1.7 | heme |
protoheme IX |
439741, 439762 |
| 1.11.1.7 | heme |
spectra are typical of high valent heme iron intermediate states generated in the catalytic cycle of a peroxidase |
742340 |
