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Information on EC 1.1.1.382 - ketol-acid reductoisomerase (NAD+) for references in articles please use BRENDA:EC1.1.1.382
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EC Tree
IUBMB Comments The enzyme, characterized from the bacteria Thermacetogenium phaeum and Desulfococcus oleovorans and from the archaeon Archaeoglobus fulgidus, is specific for NADH [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.383, ketol-acid reductoisomerase [NAD(P)+]].
The expected taxonomic range for this enzyme is: Bacteria, Archaea
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ilvC
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(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
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(2R)-2,3-dihydroxy-3-methylbutanoate:NAD+ oxidoreductase (isomerizing)
The enzyme, characterized from the bacteria Thermacetogenium phaeum and Desulfococcus oleovorans and from the archaeon Archaeoglobus fulgidus, is specific for NADH [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.383, ketol-acid reductoisomerase [NAD(P)+]].
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
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(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
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NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 13
NADH
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specific for NADH. No activity with NADPH
NADH
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specific for NADH. NADH/NADPH ratio of kcat/Km is 74
NADH
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specific for NADH. NADH/NADPH ratio of kcat/Km is 152
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additional information
NADH
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Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.005
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.032
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
1.1
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
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0.1
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.22
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.25
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.46
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
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additional information
NADH
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kcat/Km for NADH is at least 460 /mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
8
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
20
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
200
NADH
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pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
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7
assay at
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cf. EC 1.1.1.86
SwissProt
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cf. EC 1.1.1.86
SwissProt
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UniProt
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UniProt
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cf. 1.1.1.86
SwissProt
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ILVC_DESOH
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
352
0
38685
Swiss-Prot
ILVC_THEPS
Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 12270 / PB)
332
0
36963
Swiss-Prot
ILVC_UNCAG
332
0
36857
Swiss-Prot
A0A385ZLF5_9ACTN
490
0
52492
TrEMBL
ILVC_ARCFU
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
332
0
37212
Swiss-Prot
ILVC_ALIAD
Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A)
344
0
37909
Swiss-Prot
ILVC_IGNAA
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
335
0
37346
Swiss-Prot
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in complex with substrate analog L-tartaric acid, to 2.5 A resolution. The enzyme displays a six-member specificity loop, with residues Arg48 and Ser52 forming hydrogen bonds to the NAD(H) phosphate
apo-enzyme, to 1.39 A resolution, and in complex with NADP+. Enzyme accepts both cofactors NADH and NADPH. Insertions near the N-terminus of the specificity loop probably determine cofactor specificity, the laboratory engineering of NADH preference involved specific loop substitution
in complex with NADH, to 1.54 A resolution. The specificity loop differs significantly from any other crystallized ketol-acid reductoisomerases. Glu46, the first residue of the beta2alphaB-loop, lies along the N3 edge of the adenine moiety and forms a bi-dentate interaction with the O2' hydroxy of the ribose. The position of this glutamate is stabilized by a hydrogen bond interaction with Asn55
to 1.54 A resolution. Structure contains 2 Mg2+ ions and NADH. Glu46, the first residue of the beta2alphaB-loop, lies along the N3 edge of the adenine moiety and forms a bi-dentate interaction with the O2 hydroxy of the ribose. The position of this glutamate is stabilized by a hydrogen bond interaction with Asn55
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expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
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expression in Escherichia coli
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expression in Escherichia coli
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Cahn, J.K.; Brinkmann-Chen, S.; Spatzal, T.; Wiig, J.A.; Buller, A.R.; Einsle, O.; Hu, Y.; Ribbe, M.W.; Arnold, F.H.
Cofactor specificity motifs and the induced fit mechanism in Class I ketol-acid reductoisomerases
Biochem. J.
468
475-484
2015
Alicyclobacillus acidocaldarius subsp. acidocaldarius (C8WR67), Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 (C8WR67), Ignisphaera aggregans (E0SRA9), Ignisphaera aggregans DSM 17230 (E0SRA9), uncultured archaeon (Q64BR7), uncultured archaeon GZfos26G2 (Q64BR7)
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Brinkmann-Chen, S.; Cahn, J.K.; Arnold, F.H.
Uncovering rare NADH-preferring ketol-acid reductoisomerases
Metab. Eng.
26C
17-22
2014
Archaeoglobus fulgidus (O28294), Desulfococcus oleovorans, Thermacetogenium phaeum, uncultured archaeon
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1.1.1.382 ketol-acid reductoisomerase (NAD+)
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