We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme from the bacterium Castellaniella defragrans is most active in vitro with perillyl alcohol . The enzyme from the prune mite Carpoglyphus lactis also acts (more slowly) on farnesol but not on nerol .
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
GeDH,
geoA , geraniol DH,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GeDH
-
-
-
-
geoA
-
-
-
-
geraniol DH
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
geraniol + NAD+ = geranial + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
geraniol:NAD+ oxidoreductase
The enzyme from the bacterium Castellaniella defragrans is most active in vitro with perillyl alcohol [2]. The enzyme from the prune mite Carpoglyphus lactis also acts (more slowly) on farnesol but not on nerol [1].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2E,6E)-farnesol + NAD+
(2E,6E)-farnesyl aldehyde + NADH + H+
-
50% activity compared to geraniol
-
-
?
(S)-perillyl alcohol + NAD+
? + NADH + H+
(S)-perillyl alcohol + NAD+
perillyl aldehyde + NADH
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
3-methyl-but-2-enal + NADH + H+
-
4.3% activity compared to geraniol
-
-
?
benzyl alcohol + NAD+
?
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
citronellol + NAD+
?
-
-
-
?
citronellol + NAD+
citronellal + NADH + H+
cumic alcohol + NAD+
?
-
-
-
?
geraniol + dichlorophenolindophenol
?
-
-
-
?
geraniol + NAD+
geranial + NADH + H+
geraniol + oxidized dichlorophenolindophenol
geranial + reduced dichlorophenolindophenol
-
-
-
?
nerol + NAD+
neral + NADH + H+
-
-
-
?
p-cumic alcohol + NAD+
? + NADH + H+
-
-
-
?
additional information
?
-
(S)-perillyl alcohol + NAD+
? + NADH + H+
most efficient substrate
-
-
?
(S)-perillyl alcohol + NAD+
? + NADH + H+
most efficient substrate
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADH + H+
-
-
-
?
citronellol + NAD+
citronellal + NADH + H+
-
4.3% activity compared to geraniol
-
-
?
citronellol + NAD+
citronellal + NADH + H+
-
-
-
?
geraniol + NAD+
geranial + NADH + H+
-
the enzyme selectively oxidizes geraniol
-
-
ir
geraniol + NAD+
geranial + NADH + H+
-
-
-
?
geraniol + NAD+
geranial + NADH + H+
-
-
-
?
additional information
?
-
-
NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates
-
-
?
additional information
?
-
the enzyme cannot use phenazine methosulfate as alternative acceptor
-
-
?
additional information
?
-
phenazine methosulfate is not accepted as an electron acceptor
-
-
?
additional information
?
-
the enzyme cannot use phenazine methosulfate as alternative acceptor
-
-
?
additional information
?
-
phenazine methosulfate is not accepted as an electron acceptor
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
geraniol + NAD+
geranial + NADH + H+
geraniol + NAD+
geranial + NADH + H+
-
the enzyme selectively oxidizes geraniol
-
-
ir
geraniol + NAD+
geranial + NADH + H+
-
-
-
?
geraniol + NAD+
geranial + NADH + H+
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NADH
enzyme reduces one molecule of NAD+ to NADH per geraniol molecule provided
additional information
NADPH is ineffective as a cosubstrate
-
NAD+
dependent on, no activity with NADP+
NAD+
-
NADP+ is ineffective as cofactor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1-cyclohexyl-N-(2-morpholinoethyl)carbodiimide
I80 of less than 34 mM
diethylpyrocarbonate
I80 of less than 17 mM
EDTA
7 mM, 96% inhibition; 7 mM EDTA inhibits the enzyme by 96%
N-ethylmaleimide
I80 of less than 0.5 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0044 - 0.007
(S)-perillyl alcohol
0.1157 - 0.17
benzyl alcohol
0.0575 - 0.086
citronellol
0.006 - 0.021
cumic alcohol
0.0595
NAD+
-
in 50 mM Tris-HCl (pH 8.5), at 22ưC
0.021
p-cumic alcohol
pH 9.4, 21ưC
0.0044
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.007
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.007
(S)-perillyl alcohol
pH 9.4, 21ưC
0.1157
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.17
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.17
benzyl alcohol
pH 9.4, 21ưC
0.0575
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.086
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.086
citronellol
pH 9.4, 21ưC
0.006
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.021
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.0033
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.005
geraniol
pH 9.4, 21ưC
0.05
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.051
geraniol
-
in 50 mM Tris-HCl (pH 8.5), at 22ưC
0.0232
nerol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.045
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
0.045
nerol
pH 9.4, 21ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2996
geraniol
-
in 50 mM Tris-HCl (pH 8.5), at 22ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2020 - 3550
(S)-perillyl alcohol
520
p-cumic alcohol
pH 9.4, 21ưC
2020
(S)-perillyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
2020
(S)-perillyl alcohol
pH 9.4, 21ưC
3550
(S)-perillyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
110
benzyl alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
220
benzyl alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
220
benzyl alcohol
pH 9.4, 21ưC
50
citronellol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
100
citronellol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
100
citronellol
pH 9.4, 21ưC
520
cumic alcohol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
940
cumic alcohol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
620
geraniol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
1570
geraniol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
1570
geraniol
pH 9.4, 21ưC
58800
geraniol
-
in 50 mM Tris-HCl (pH 8.5), at 22ưC
310
nerol
native enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
310
nerol
recombinant enzyme, in 100 mM glycine-NaOH, pH 9.4, at 21ưC
310
nerol
pH 9.4, 21ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.016
-
crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22ưC
31
-
after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22ưC
additional information
the crude cell extract shows a specific activity of 0.056 units/mg, while the 114fold purified enzyme shows a specific activity of 4.33 units/mg at pH 9.4 and 21ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 10
-
as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
UniProt
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GEDH_CARLC
378
0
40630
Swiss-Prot
other Location (Reliability: 5 )
ADH2_TANCI
379
0
41397
Swiss-Prot
other Location (Reliability: 3 )
GEOA_CASDE
373
0
38274
Swiss-Prot
-
A0A2Z3YPQ3_9CORY
372
0
38803
TrEMBL
-
A0A645IZ68_9ZZZZ
65
0
7108
TrEMBL
other Location (Reliability: 2 )
A0A1D7ZZ31_LIMFE
361
0
38608
TrEMBL
-
A0A2P7ZBE2_9ACTN
363
0
37289
TrEMBL
-
A0A231GXB6_9NOCA
367
0
37434
TrEMBL
-
A0A7K0D4G2_9NOCA
363
0
37163
TrEMBL
-
A0A231H3F7_9NOCA
360
1
36774
TrEMBL
-
A0A084FZJ5_PSEDA
342
0
36497
TrEMBL
other Location (Reliability: 2 )
A3KI51_STRA7
Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516)
368
2
38255
TrEMBL
-
A0A653LD66_9MICC
367
0
38295
TrEMBL
-
A0A2I2KPZ4_9ACTN
367
0
37260
TrEMBL
-
A0A0X8R1M5_9SPHN
375
0
39060
TrEMBL
-
A0A1V5EDA2_9DELT
75
0
8521
TrEMBL
-
A0A1D7ZZ49_LIMFE
356
0
38453
TrEMBL
-
A0A1V4XST1_9DELT
75
0
8521
TrEMBL
-
A0A0J6VIH5_9MYCO
362
2
36855
TrEMBL
-
A0A5E7UY72_PSEFL
374
0
38612
TrEMBL
-
A0A2S8BNS4_9MYCO
149
0
15885
TrEMBL
-
A0A7K0DJC2_9NOCA
361
0
36473
TrEMBL
-
A0A7W9WQ58_CASDE
373
0
38256
TrEMBL
-
A0A0M2H8A0_9MICO
534
0
55492
TrEMBL
-
A0A0K2YIV5_9NOCA
373
1
37894
TrEMBL
-
A0A330LDK8_LACSK
139
0
15215
TrEMBL
-
A0A7X5XRS7_9SPHN
366
2
37586
TrEMBL
-
A0A7S4LVC1_9GAMM
346
0
36613
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
39000
2 * 39000, SDS-PAGE
40630
-
1 * 40630, calculated from amino acid sequence
42800
-
1 * 42800, active form, SDS-PAGE
52000 - 91000
gel filtration
38272
2 * 38272, calculated from amino acid sequence
38272
2 * 38272, calculated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
2 * 38272, calculated
dimer
-
2 * 38272, calculated
-
homodimer
2 * 39000, SDS-PAGE
homodimer
2 * 38272, calculated from amino acid sequence
homodimer
-
2 * 39000, SDS-PAGE
-
homodimer
-
2 * 38272, calculated from amino acid sequence
-
monomer
-
1 * 40630, calculated from amino acid sequence
monomer
-
1 * 42800, active form, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration
-
DEAE column chromatography and phenyl-Sepharose 6 gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli strain BL21 Star(DE3)
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
activitiy is induced during growth with limonene in comparison to growth with acetate
enzyme activity is induced during growth with limonene in comparison to growth with acetate
activitiy is induced during growth with limonene in comparison to growth with acetate
activitiy is induced during growth with limonene in comparison to growth with acetate
-
-
enzyme activity is induced during growth with limonene in comparison to growth with acetate
enzyme activity is induced during growth with limonene in comparison to growth with acetate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.
Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae)
FEBS J.
275
2807-2817
2008
Carpoglyphus lactis
brenda
Lueddeke, F.; Wuelfing, A.; Timke, M.; Germer, F.; Weber, J.; Dikfidan, A.; Rahnfeld, T.; Linder, D.; Meyerdierks, A.; Harder, J.
Geraniol and geranial dehydrogenases induced in anaerobic monoterpene degradation by Castellaniella defragrans
Appl. Environ. Microbiol.
78
2128-2136
2012
Castellaniella defragrans (H1ZV38), Castellaniella defragrans 65Phen (H1ZV38)
brenda
Select items on the left to see more content.
html completed