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Enzyme Nomenclature
Information on EC 1.1.1.341 - CDP-abequose synthase
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1.1.1.341 CDP-abequose synthaseIUBMB Comments
Isolated from Yersinia pseudotuberculosis [1,3] and Salmonella enterica [1,2].
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
rfbJ
rfbJ
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CDP-alpha-D-abequose + NADP+ = CDP-4-dehydro-3,6-dideoxy-alpha-D-glucose + NADPH + H+
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
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SYSTEMATIC NAME
IUBMB Comments
CDP-alpa-D-abequose:NADP+ 4-oxidoreductase
Isolated from Yersinia pseudotuberculosis [1,3] and Salmonella enterica [1,2].
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
enzyme catalyzes the last step in synthesis of CDP-abequose from CPD-glucose. The 3,6-dideoxyhexose biosynthetic genes are clustered and relatively conserved throughout all four serogroups of Yersinia tuberculosis
physiological function
enzyme is involved in synthesis of abequose, a constituent of the O-antigen of Salmonella B group strains
physiological function
-
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results suggest that the development of the 0-antigen serotype diversity of Salmonella is probably the result of both sequence divergence and recombination between heterologous rfb sequences. In addition, the results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
physiological function
-
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results suggest that the development of the 0-antigen serotype diversity of Salmonella is probably the result of both sequence divergence and recombination between heterologous rfb sequences. In addition, the results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). RFBJ_SALTY
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
299
0
34106
Swiss-Prot
-
A0A1X9MAS3_9BACI
312
0
35543
TrEMBL
-
A0A645BRZ1_9ZZZZ
302
0
33526
TrEMBL
Secretory Pathway (Reliability: 3)
A0A607YAJ2_SALET
293
0
33747
TrEMBL
-
A0A345DC29_9BURK
306
0
34428
TrEMBL
-
A0A1J5R1G0_9ZZZZ
291
0
32368
TrEMBL
Mitochondrion (Reliability: 4)
A0A1J5PF50_9ZZZZ
358
0
39432
TrEMBL
Secretory Pathway (Reliability: 5)
A0A259UEJ9_9FIRM
295
0
33031
TrEMBL
-
A0A2S6TPN1_9PROT
293
0
33294
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
33000
x * 33000, SDS-PAGE, x * 34106, calculated
34106
x * 33000, SDS-PAGE, x * 34106, calculated
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 33000, SDS-PAGE, x * 34106, calculated
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
selection and use of primers to target defined regions of the abequose and paratose synthase genes responsible for biosynthesis of the oligosaccharide repeating units of the 0-antigenic lipopolysaccharide, in order to differentiate Salmonella serogroups. In a polymerase chain reaction assay utilizing these rjb-specific primers, all of the 40 salmonellae belonging to serogroups B, C2, and D plus A could accurately be identified among a total of 123 clinical isolates tested. No false-positive reactions were detected
medicine
medicine
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insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
medicine
-
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wyk, P.; Reeves, P.
Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase
J. Bacteriol.
171
5687-5693
1989
Salmonella enterica subsp. enterica serovar Typhimurium (P0A1P4)
brenda
Thorson, J.; Lo, S.; Ploux, O.; He, X.; Liu, H.
Studies of the biosynthesis of 3,6-dideoxyhexoses: Molecular cloning and characterization of the asc (ascarylose) region from Yersinia pseudotuberculosis serogroup VA
J. Bacteriol.
176
5483-5493
1994
Yersinia pseudotuberculosis
brenda
Luk, J.M.; Kongmuang, U.; Reeves, P.R.; Lindberg, A.A.
Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)
J. Clin. Microbiol.
31
2118-2123
1993
Salmonella enterica subsp. enterica serovar Typhimurium (P0A1P4)
brenda
Kessler, A.C.; Brown, P.K.; Romana, L.K.; Reeves, P.R.
Molecular cloning and genetic characterization of the rfb region from Yersinia pseudotuberculosis serogroup IIA, which determines the formation of the 3,6-dideoxyhexose abequose
J. Gen. Microbiol.
137
2689-2695
1991
Yersinia pseudotuberculosis (Q05342)
brenda
Hone, D.M.; Harris, A.M.; Lim, V.; Levine, M.M.
Construction and characterization of isogenic O-antigen variants of Salmonella typhi
Mol. Microbiol.
13
525-530
1994
Salmonella enterica subsp. enterica serovar Typhi, Salmonella enterica subsp. enterica serovar Typhi LT2
brenda
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