enzyme catalyzes the last step in synthesis of CDP-abequose from CPD-glucose. The 3,6-dideoxyhexose biosynthetic genes are clustered and relatively conserved throughout all four serogroups of Yersinia tuberculosis
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results suggest that the development of the 0-antigen serotype diversity of Salmonella is probably the result of both sequence divergence and recombination between heterologous rfb sequences. In addition, the results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results suggest that the development of the 0-antigen serotype diversity of Salmonella is probably the result of both sequence divergence and recombination between heterologous rfb sequences. In addition, the results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
selection and use of primers to target defined regions of the abequose and paratose synthase genes responsible for biosynthesis of the oligosaccharide repeating units of the 0-antigenic lipopolysaccharide, in order to differentiate Salmonella serogroups. In a polymerase chain reaction assay utilizing these rjb-specific primers, all of the 40 salmonellae belonging to serogroups B, C2, and D plus A could accurately be identified among a total of 123 clinical isolates tested. No false-positive reactions were detected
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
insertion of the gene rfbJ encoding abequose synthase into a suicide vector and integration into the rfb clusters of wild-type Salmonella typhi Vi-positive strain ISP 1820, Salmonella typhi Vi-negative strain H400, and a double aro mutant of Salmonella typhi ISP 1820, strain CVD 906. Strains carrying the vector express the O4 antigen in place of the O9 antigen in the lipopolysaccharide molecule. Results support the hypothesis that the chemical composition of the Salmonella 0-antigen influences the interaction of individual serotypes with complement
Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase
Studies of the biosynthesis of 3,6-dideoxyhexoses: Molecular cloning and characterization of the asc (ascarylose) region from Yersinia pseudotuberculosis serogroup VA
Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D)
Molecular cloning and genetic characterization of the rfb region from Yersinia pseudotuberculosis serogroup IIA, which determines the formation of the 3,6-dideoxyhexose abequose
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Enzyme Nomenclature
1.1.1.341 CDP-abequose synthase
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