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Information on EC 1.1.1.138 - mannitol 2-dehydrogenase (NADP+) and Organism(s) Alternaria alternata and UniProt Accession P0C0Y4

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This record set is specific for:
Alternaria alternata
UNIPROT: P0C0Y4 not found.
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Word Map
The taxonomic range for the selected organisms is: Alternaria alternata
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
mad1p, d-mannitol dehydrogenase, cla h 8, psldr, gox1432, nadp-dependent mannitol dehydrogenase, d-mannitol 2-dehydrogenase, tbmdh, lrmdh, nadp-mannitol dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADP-dependent mannitol dehydrogenase
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Mannitol 2-dehydrogenase [NADP+]
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mannitol dehydrogenase
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mannitol dehydrogenase (NADP+)
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mannitol dehydrogenase (nicotinamide dinucleotide phosphate)
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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-
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reduction
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
D-mannitol:NADP+ 2-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-68-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-fructose + NADPH + H+
D-mannitol + NADP+
show the reaction diagram
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-
-
r
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
show the reaction diagram
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-
-
-
?
D-mannitol + NADP+
D-fructose + NADPH + H+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol + NADP+
D-fructose + NADPH + H+
show the reaction diagram
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involved in the mannitol cycle, important for NADPH regeneration
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose
substrate inhibition occurred at concentrations above 750 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
474
D-fructose
0.0187
NADPH
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MTDH_ALTAL
266
0
28735
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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MtDH mutant produces less mannitol than compared to the wild-type. The MtDH/MPDH double mutant does not produce mannitol. Response of the genes in the disruption mutants is similar to that in wild-type. Conidia of mutants are viable and germinated normally. Elevated levels of trehalose in the mutants, suggesting that trehalose may substitute for mannitol as a storage compound for germination. Tobacco inoculation shows no reduction in lesion severity caused by the MtDH mutant as compared with wild-type, whereas the MtDH/MPDH double mutant causes significantly less disease. The double mutant is unaffected in the ability to germinate and produce appressoria on tobacco leaves and elicites a defense response from the host, indicating that it is able to penetrate and infect the host
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hult, K.; Veide, A.; Gatenbeck, S.
The distribution of the NADPH regenerating mannitol cycle among fungal species
Arch. Microbiol.
128
253-255
1980
Alternaria alternata, Botrytis cinerea, Ceratocystis multiannulata, Cladosporium cladosporioides, Fusarium graminearum, Neurospora crassa, Penicillium glabrum, Talaromyces islandicus, Thermomyces lanuginosus
Manually annotated by BRENDA team
Schneider, P.B.; Denk, U.; Breitenbach, M.; Richter, K.; Schmid-Grendelmeier, P.; Nobbe, S.; Himly, M.; Mari, A.; Ebner, C.; Simon-Nobbe, B.
Alternaria alternata NADP-dependent mannitol dehydrogenase is an important fungal allergen
Clin. Exp. Allergy
36
1513-1524
2006
Alternaria alternata (P0C0Y4), Alternaria alternata
Manually annotated by BRENDA team
Velez, H.; Glassbrook, N.J.; Daub, M.E.
Mannitol biosynthesis is required for plant pathogenicity by Alternaria alternata
FEMS Microbiol. Lett.
285
122-129
2008
Alternaria alternata, Alternaria alternata A5
Manually annotated by BRENDA team