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EC 3.5.1.54 Details
EC number
3.5.1.54
Accepted name
allophanate hydrolase
Reaction
urea-1-carboxylate + H2O = 2 CO2 + 2 NH3
Other name(s)
allophanate lyase, AtzF, TrzF
Systematic name
urea-1-carboxylate amidohydrolase
CAS registry number
9076-72-6
Comment
Along with EC 3.5.2.15 (cyanuric acid amidohydrolase) and EC 3.5.1.84 (biuret amidohydrolase), this enzyme forms part of the cyanuric-acid metabolism pathway, which degrades s-triazide herbicides, such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine], in bacteria. The yeast enzyme (but not that from green algae) also catalyses the reaction of EC 6.3.4.6, urea carboxylase, thus bringing about the hydrolysis of urea to CO2 and NH3 in the presence of ATP and bicarbonate. The enzyme from Pseudomonas sp. strain ADP has a narrow substrate specificity, being unable to use the structurally analogous compounds urea, hydroxyurea or methylcarbamate as substrate [6].
History
created 1986, modified 2008
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EC Tree
1 Oxidoreductases
2 Transferases
3 Hydrolases
3.1 Acting on ester bonds
3.2 Glycosylases
3.3 Acting on ether bonds
3.4 Acting on peptide bonds (peptidases)
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.5.1 In linear amides
3.5.1.1 asparaginase
3.5.1.2 glutaminase
3.5.1.3 ω-amidase
3.5.1.4 amidase
3.5.1.5 urease
3.5.1.6 β-ureidopropionase
3.5.1.7 ureidosuccinase
3.5.1.8 formylaspartate deformylase
3.5.1.9 arylformamidase
3.5.1.10 formyltetrahydrofolate deformylase
3.5.1.11 penicillin amidase
3.5.1.12 biotinidase
3.5.1.13 aryl-acylamidase
3.5.1.14 N-acyl-aliphatic-L-amino acid amidohydrolase
3.5.1.15 aspartoacylase
3.5.1.16 acetylornithine deacetylase
3.5.1.17 acyl-lysine deacylase
3.5.1.18 succinyl-diaminopimelate desuccinylase
3.5.1.19 nicotinamidase
3.5.1.20 citrullinase
3.5.1.21 N-acetyl-β-alanine deacetylase
3.5.1.22 pantothenase
3.5.1.23 ceramidase
3.5.1.24 choloylglycine hydrolase
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
3.5.1.26 N4-(β-N-acetylglucosaminyl)-L-asparaginase
3.5.1.27 N-formylmethionylaminoacyl-tRNA deformylase
3.5.1.28 N-acetylmuramoyl-L-alanine amidase
3.5.1.29 2-(acetamidomethylene)succinate hydrolase
3.5.1.30 5-aminopentanamidase
3.5.1.31 formylmethionine deformylase
3.5.1.32 hippurate hydrolase
3.5.1.33 N-acetylglucosamine deacetylase
3.5.1.34 created 1972, deleted 1981
3.5.1.35 D-glutaminase
3.5.1.36 N-methyl-2-oxoglutaramate hydrolase
3.5.1.37 created 1972, deleted 1976
3.5.1.38 glutamin-(asparagin-)ase
3.5.1.39 alkylamidase
3.5.1.40 acylagmatine amidase
3.5.1.41 chitin deacetylase
3.5.1.42 nicotinamide-nucleotide amidase
3.5.1.43 peptidyl-glutaminase
3.5.1.44 protein-glutamine glutaminase
3.5.1.45 created 1978, deleted 1986
3.5.1.46 6-aminohexanoate-oligomer exohydrolase
3.5.1.47 N-acetyldiaminopimelate deacetylase
3.5.1.48 acetylspermidine deacetylase
3.5.1.49 formamidase
3.5.1.50 pentanamidase
3.5.1.51 4-acetamidobutyryl-CoA deacetylase
3.5.1.52 peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase
3.5.1.53 N-carbamoylputrescine amidase
3.5.1.54 allophanate hydrolase
3.5.1.55 long-chain-fatty-acyl-glutamate deacylase
3.5.1.56 N,N-dimethylformamidase
3.5.1.57 tryptophanamidase
3.5.1.58 N-benzyloxycarbonylglycine hydrolase
3.5.1.59 N-carbamoylsarcosine amidase
3.5.1.60 N-(long-chain-acyl)ethanolamine deacylase
3.5.1.61 mimosinase
3.5.1.62 acetylputrescine deacetylase
3.5.1.63 4-acetamidobutyrate deacetylase
3.5.1.64 Nα-benzyloxycarbonylleucine hydrolase
3.5.1.65 theanine hydrolase
3.5.1.66 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
3.5.1.67 4-methyleneglutaminase
3.5.1.68 N-formylglutamate deformylase
3.5.1.69 glycosphingolipid deacylase
3.5.1.70 aculeacin-A deacylase
3.5.1.71 N-feruloylglycine deacylase
3.5.1.72 D-benzoylarginine-4-nitroanilide amidase
3.5.1.73 carnitinamidase
3.5.1.74 chenodeoxycholoyltaurine hydrolase
3.5.1.75 urethanase
3.5.1.76 arylalkyl acylamidase
3.5.1.77 N-carbamoyl-D-amino-acid hydrolase
3.5.1.78 glutathionylspermidine amidase
3.5.1.79 phthalyl amidase
3.5.1.80 created 1999, deleted 2002
3.5.1.81 N-acyl-D-amino-acid deacylase
3.5.1.82 N-acyl-D-glutamate deacylase
3.5.1.83 N-acyl-D-aspartate deacylase
3.5.1.84 biuret amidohydrolase
3.5.1.85 (S)-N-acetyl-1-phenylethylamine hydrolase
3.5.1.86 mandelamide amidase
3.5.1.87 N-carbamoyl-L-amino-acid hydrolase
3.5.1.88 peptide deformylase
3.5.1.89 N-acetylglucosaminylphosphatidylinositol deacetylase
3.5.1.90 adenosylcobinamide hydrolase
3.5.1.91 N-substituted formamide deformylase
3.5.1.92 pantetheine hydrolase
3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylase
3.5.1.94 γ-glutamyl-γ-aminobutyrate hydrolase
3.5.1.95 N-malonylurea hydrolase
3.5.1.96 succinylglutamate desuccinylase
3.5.1.97 acyl-homoserine-lactone acylase
3.5.1.98 histone deacetylase
3.5.1.99 fatty acid amide hydrolase
3.5.1.100 (R)-amidase
3.5.1.101 L-proline amide hydrolase
3.5.1.102 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5′-monophosphate deformylase
3.5.1.103 N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-α-D-glucopyranoside deacetylase
3.5.1.104 peptidoglycan-N-acetylglucosamine deacetylase
3.5.1.105 chitin disaccharide deacetylase
3.5.1.106 N-formylmaleamate deformylase
3.5.1.107 maleamate amidohydrolase
3.5.1.108 UDP-3-O-acyl-N-acetylglucosamine deacetylase
3.5.1.109 sphingomyelin deacylase
3.5.1.110 ureidoacrylate amidohydrolase
3.5.1.111 2-oxoglutaramate amidase
3.5.1.112 2′-N-acetylparomamine deacetylase
3.5.1.113 2′′′-acetyl-6′′′-hydroxyneomycin C deacetylase
3.5.1.114 N-acyl-aromatic-L-amino acid amidohydrolase
3.5.1.115 mycothiol S-conjugate amidase
3.5.1.116 ureidoglycolate amidohydrolase
3.5.1.117 6-aminohexanoate-oligomer endohydrolase
3.5.1.118 γ-glutamyl hercynylcysteine S-oxide hydrolase
3.5.1.119 Pup amidohydrolase
3.5.1.120 2-aminomuconate deaminase (2-hydroxymuconate-forming)
3.5.1.121 protein N-terminal asparagine amidohydrolase
3.5.1.122 protein N-terminal glutamine amidohydrolase
3.5.1.123 γ-glutamylanilide hydrolase
3.5.1.124 protein deglycase
3.5.1.125 N2-acetyl-L-2,4-diaminobutanoate deacetylase
3.5.1.126 oxamate amidohydrolase
3.5.1.127 jasmonoyl-L-amino acid hydrolase
3.5.1.128 deaminated glutathione amidase
3.5.1.129 N5-(cytidine 5′-diphosphoramidyl)-L-glutamine hydrolase
3.5.1.130 [amino group carrier protein]-lysine hydrolase
3.5.1.131 1-carboxybiuret hydrolase
3.5.1.132 [amino group carrier protein]-ornithine hydrolase
3.5.1.133 Nα-acyl-L-glutamine aminoacylase
3.5.1.134 (indol-3-yl)acetyl-L-aspartate hydrolase
3.5.1.135 N4-acetylcytidine amidohydrolase
3.5.1.136 N,N′-diacetylchitobiose non-reducing end deacetylase
3.5.1.137 N-methylcarbamate hydrolase
3.5.2 In cyclic amides
3.5.3 In linear amidines
3.5.4 In cyclic amidines
3.5.5 In nitriles
3.5.99 In other compounds
3.6 Acting on acid anhydrides
3.7 Acting on carbon-carbon bonds
3.8 Acting on halide bonds
3.9 Acting on phosphorus-nitrogen bonds
3.10 Acting on sulfur-nitrogen bonds
3.11 Acting on carbon-phosphorus bonds
3.12 Acting on sulfur-sulfur bonds
3.13 Acting on carbon-sulfur bonds
4 Lyases
5 Isomerases
6 Ligases
7 Translocases