1.2.3.15: (methyl)glyoxal oxidase

This is an abbreviated version!
For detailed information about (methyl)glyoxal oxidase, go to the full flat file.

Reaction

Synonyms

GLOX, Glox2, Glox3, GLX, Glx1, GLX2, methylglyoxal oxidase, MYCTH_2294895

ECTree

1 Oxidoreductases

1.2 Acting on the aldehyde or oxo group of donors

1.2.3 With oxygen as acceptor

1.2.3.15 (methyl)glyoxal oxidase

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Results	in table
1	Activating Compound
10	AA Sequence
1	Application
3	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
1	Inhibitors
11	kcat/KM [mM/s]
2	Ki Value [mM]
13	KM Value [mM]
5	Metals/Ions
3	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
28	Organism
4	pH Optimum
2	pH Range
5	pI Value
2	Posttranslational Modification
2	Purification (Commentary)
2	Reaction
16	Reference
45	Substrates and Products (Substrate)
7	Subunits
25	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Application

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Application on EC 1.2.3.15 - (methyl)glyoxal oxidase

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synthesis

Phanerodontia chrysosporium

Q01772

efficient expression in Pichia pastoris and Trichoderma reesei with subsequent purification by anion exchange and hydrophobic interaction chromatography. Both processes are suitable for the production of the target protein at high levels. GLOX produced in T. reesei carries mainly Man5 glycosylation while the enzyme produced in P. pastoris exhibits the typical high-mannose type N-glycosylation. The enzyme expressed in P. pastoris shows slightly higher specific activities which correlates with the higher copper loading of 65.5 % compared to 51.9 % for the protein from T. reesei

762988