1.2.1.105: 2-oxoglutarate dehydrogenase system
This is an abbreviated version!
For detailed information about 2-oxoglutarate dehydrogenase system, go to the full flat file.
Word Map on EC 1.2.1.105
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1.2.1.105
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kgdhc
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dihydrolipoamide
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thiamin
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alpha-ketoacids
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2-oxoadipate
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transsuccinylase
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charcot-marie-tooth
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2-aminoadipic
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medicine
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thiokinase
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glutaryl-coa
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analysis
- 1.2.1.105
- kgdhc
- dihydrolipoamide
- thiamin
- alpha-ketoacids
- 2-oxoadipate
-
transsuccinylase
- charcot-marie-tooth
-
2-aminoadipic
- medicine
- thiokinase
- glutaryl-coa
- analysis
Reaction
Synonyms
2-OGDH2, 2-oxoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase complex, alpha-KDE2, alpha-ketoglutarate dehydrogenase, alpha-ketoglutarate dehydrogenase complex, alpha-KGDH, At3g55410, At5g65750, DHTKD1, dihydrolipoyl succinyltransferase E2, E1a, E1k, E1o, E2, KGDH, KGDHC, More, MPA24.10, ODGH, ODGH1, ODGH2, ODH, OGDC, OGDH, OGDHC, OGDHL, OGHDC-E2, PDHC, SucA, SucB
ECTree
Advanced search results
KM Value
KM Value on EC 1.2.1.105 - 2-oxoglutarate dehydrogenase system
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0.52
2-oxoadipate
recombinant enzyme complex, pH 7.5, 37°C
0.0025
mutant H298T, pH not specified in the publication, temperature not specified in the publication
0.00261
2-oxoglutarate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.00273
2-oxoglutarate
mutant H260E/H298N, pH not specified in the publication, temperature not specified in the publication
0.00341
2-oxoglutarate
mutant H298L, pH not specified in the publication, temperature not specified in the publication
0.06
2-oxoglutarate
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overall activity of the complex, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
0.08
2-oxoglutarate
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activity of component E1, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
0.11
2-oxoglutarate
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overall activity of the complex, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
0.13
2-oxoglutarate
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activity of component E1, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
0.15
2-oxoglutarate
activity of component E1, presence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
0.15
2-oxoglutarate
recombinant enzyme complex, pH 7.5, 37°C
0.165
2-oxoglutarate
mutant H298V, pH not specified in the publication, temperature not specified in the publication
0.2
2-oxoglutarate
activity of component E1, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
0.21
2-oxoglutarate
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activity of component E1, presence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
0.251
2-oxoglutarate
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S0.5 value, pH 7.5, temperature not specified in the publication
0.28
2-oxoglutarate
activity of component E1, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
0.29
2-oxoglutarate
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overall reaction, enzyme from euthermic animals, 22°C, pH 7.8
0.29
2-oxoglutarate
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overall reaction, enzyme from hibernating animals, 37°C, pH 7.8
0.32
2-oxoglutarate
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overall reaction, enzyme from euthermic animals, 37°C, pH 7.8
0.38
2-oxoglutarate
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overall reaction, enzyme from hibernating animals, 22°C, pH 7.8
0.383
2-oxoglutarate
mutant H260E, pH not specified in the publication, temperature not specified in the publication
0.4
2-oxoglutarate
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overall reaction, enzyme from hibernating animals, 5°C, pH 7.8
0.45
2-oxoglutarate
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overall reaction, enzyme from euthermic animals, 5°C, pH 7.8
1.28
2-oxoglutarate
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overall activity of the complex, absence of Ca2+, absence of NADH, 30°C, pH not specified in the publication
1.48
2-oxoglutarate
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activity of component E1, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
1.57
2-oxoglutarate
activity of component E1, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
10.1
2-oxoglutarate
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overall activity of the complex, absence of Ca2+, presence of NADH, 30°C, pH not specified in the publication
0.0063
mutant H298L, pH not specified in the publication, temperature not specified in the publication
0.00702
2-oxovalerate
mutant H298D, pH not specified in the publication, temperature not specified in the publication
0.00896
2-oxovalerate
mutant H298V, pH not specified in the publication, temperature not specified in the publication
0.0153
2-oxovalerate
mutant H298T, pH not specified in the publication, temperature not specified in the publication
0.0163
2-oxovalerate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.023
CoA
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overall reaction, enzyme from euthermic animals, 22°C, pH 7.8
0.026
CoA
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overall reaction, enzyme from euthermic animals, 37°C, pH 7.8
0.028
CoA
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overall reaction, enzyme from euthermic animals, 5°C, pH 7.8
0.065
CoA
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overall reaction, enzyme from hibernating animals, 37°C, pH 7.8
0.067
CoA
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overall reaction, enzyme from hibernating animals, 5°C, pH 7.8
0.075
CoA
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overall reaction, enzyme from hibernating animals, 22°C, pH 7.8
0.0249
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S0.5 value, Hill coefficient 0.4, pH 7.5, temperature not specified in the publication
0.09
NAD+
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overall reaction, enzyme from hibernating animals, 5°C, pH 7.8
0.11
NAD+
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overall reaction, enzyme from euthermic animals, 5°C, pH 7.8
0.17
NAD+
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overall reaction, enzyme from hibernating animals, 37°C, pH 7.8
0.19
NAD+
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overall reaction, enzyme from euthermic animals, 22°C, pH 7.8
0.25
NAD+
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overall reaction, enzyme from euthermic animals, 37°C, pH 7.8
0.3
NAD+
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overall reaction, enzyme from hibernating animals, 22°C, pH 7.8
additional information
additional information
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two substrate-binding modes are revealed at different degrees of saturation of the enzyme with 2-oxoglutarate. At low substrate concentrations, 0.001-0.01 mM, the binding mainly depends on the interaction of the enzyme with the substrate carbonyl groups. At 0.1-1 mM the relative contribution of the 2-oxo group in the binding increases
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additional information
additional information
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kinetic study, reaction of 2-oxoglutarate dehydrogenase complex
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additional information
additional information
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the Hill coefficient for 2-oxoglutarate and NAD at 0.2 M ionic strength is 1.0, whereas at 0.05 M ionic strength it is 0.85 and 1.2 for 2-oxoglutarate and NAD, respectively, pH 7.8, 30°C
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