EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.62 | H370A |
mutant with less than 5% of activity of wild-type enzyme |
646438 |
| 3.1.3.62 | H89A |
catalytically compromised mutant of MIPP1 that shows only 1% of the 2,3-diphosphoglycerate phosphatase activity of the wild type enzyme |
694894 |
| 3.1.3.62 | more |
L1ALP contains the histidine phosphatase conserved sequence, -RHGXRXP-, and therefore belongs to the histidine phosphatase family of enzymes unlike alkaline phytases from bacteria |
682291 |
| 3.1.3.62 | more |
mutant with active site histidine altered to alanine has abolished enzyme activity |
646439 |
| 3.1.3.62 | more |
two isoforms of alkaline phytase cDNAs, L1Alp1 and L1Alp2, which are 1467 and 1533 bp long and encode proteins of 487 and 511 amino acids, respectively, are identified. Phylogenetic analysis reveals that LlALP is most closely related to multiple inositol polyphosphate phosphatase (MINPP) from humans (25%) and rats (23%) |
682291 |
| 3.1.3.62 | Q78A |
Km: 0.06 (myo-inositol hexakisphosphate). Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other organic phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, ribose 1-phosphate: 3% |
680964 |
| 3.1.3.62 | T27G |
Km: 0.092 (myo-inositol hexakisphosphate) |
680964 |
