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Literature summary for 3.1.3.62 extracted from

  • Cho, J.; Choi, K.; Darden, T.; Reynolds, P.R.; Petitte, J.N.; Shears, S.B.
    Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planets "phosphate crisis" (2006), J. Biotechnol., 126, 248-259.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.62

Application

Application Comment Organism
biotechnology recombinant avian MINPP (0.7 micromol/mg protein/min) is the most active phytase found to date in any animal cell Gallus gallus

Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding residues 19-445 of avian MINPP or residues 31-483 of human MINPP lacking the N-terminal ER targeting sequences and the C-terminal ER retention signals are amplified by PCR and cloned into pGEM Tvector and transformed into Escherichia coli Gallus gallus

Protein Variants

Protein Variants Comment Organism
Q78A Km: 0.06 (myo-inositol hexakisphosphate). Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other organic phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, ribose 1-phosphate: 3% Gallus gallus
T27G Km: 0.092 (myo-inositol hexakisphosphate) Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 myo-inositol hexakisphosphate mutant Q78A Gallus gallus
0.092
-
 myo-inositol hexakisphosphate mutant T27G Gallus gallus
0.14
-
 myo-inositol hexakisphosphate
-
 Gallus gallus
1.74
-
 scyllo-inositol hexakisphosphate
-
 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 avian
-

Purification (Commentary)

Purification (Comment) Organism
purification is carried out on a Ni-sepharose column Gallus gallus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of MINPP towards other phosphates, relative to inositol hexakisphosphate, is as follows: p-nitrophenyl phosphate: 17%, ATP: 11%, D-ribose 1-phosphate: 0.31% Gallus gallus
additional information
-
 Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, D-ribose 1-phosphate: 3% Gallus gallus
0.7
-
 specific activity of recombinant avian MINPP Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Gallus gallus myo-inositol pentakisphosphate + phosphate
-
 ?
scyllo-inositol hexakisphosphate + H2O
-
 Gallus gallus scyllo-inositol pentakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Minpp
-
 Gallus gallus
multiple inositol polyphosphate phosphatase
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Gallus gallus