EC Number |
Protein Variants |
Reference |
---|
2.3.1.21 | A305C |
site-directed mutagenesis, the revertant mutant shows about wild-type activity |
674520 |
2.3.1.21 | A381D |
site-directed mutagenesis, activity is reduced by 86%, Km for acyl-CoA is 6-8fold increased |
486309 |
2.3.1.21 | A478G |
3.2fold increase in IC50 for malonyl-CoA, 2.6fold increase in KM-value for carnitine, 3.1fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme |
659306 |
2.3.1.21 | A478G |
site-directed mutagenesis, the mutant shows decreased sensitivity to malonyl-CoA compared to the wild-type enzyme |
674818 |
2.3.1.21 | A587S/S588A/M592L/R594L |
inert isoform CPT1c |
735458 |
2.3.1.21 | C304A |
reduced expression in COS-7 cell, reduced activity |
702112 |
2.3.1.21 | C304W |
reduced expression in COS-7 cell, reduced activity |
702112 |
2.3.1.21 | C305A |
5% of wild-type activity in mitochondrial fraction, 30% of wild-type expression in COS-7 cell |
702112 |
2.3.1.21 | C305A |
site-directed mutagenesis, inactive mutant |
674520 |
2.3.1.21 | C305D |
73% of wild-type activity in mitochondrial fraction (COS-7 cell), 81% of wild-type expression in COS-7 cell |
702112 |