Literature summary for 2.3.1.21 extracted from

Morillas, M.; Gomez-Puertas, P.; Bentebibel, A.; Selles, E.; Casals, N.; Valencia, A.; Hegardt, F.G.; Asins, G.; Serra, D.
Identification of conserved amino acid residues in rat liver carnitine palmitoyltransferase I critical for malonyl-CoA inhibition (2003), J. Biol. Chem., 278, 9058-9063.

Search for more literature for 2.3.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression of carnitine palmitoyltransferase I wild-type and mutant enzymes in Saccharomyces cerevisiae	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
A478G	3.2fold increase in IC50 for malonyl-CoA, 2.6fold increase in KM-value for carnitine, 3.1fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
C608A	2.2fold increase in IC50 for malonyl-CoA, 2.5fold increase in KM-value for carnitine, 5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
M593A	12.6fold increase in IC50 for malonyl-CoA, 2.3fold increase in KM-value for carnitine, 1.2fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
M593E	18fold increase in IC50 for malonyl-CoA, 1.2fold increase in KM-value for carnitine, 1.3fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
M593S	26fold increase in IC50 for malonyl-CoA, KM-value for carnitine is nearly identical to wild-type value, 1.5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
N464D	1.4fold decrease in IC50 for malonyl-CoA, 1.8fold increase in KM-value for carnitine, 1.2fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus
T314S	1.2fold increase in IC50 for malonyl-CoA, 1.4fold increase in KM-value for carnitine, 2.9fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
malonyl-CoA	IC50: 0.0123 for wild-type enzyme, 0.015 mM for mutant enzyme T314S, 0.0087 mM for mutant enzyme N464D, 0.0395 mM for mutant enzyme A478G, 0.0275 mM for mutant enzyme C608A, 0.319 mM for mutant enzyme M593 mM, 0.155 mM for mutant enzyme M593A, 0.22 mM for mutant enzyme M593E	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0017	-	palmitoyl-CoA	mutant T314S	Rattus norvegicus
0.0017	-	L-carnitine	mutant T314S	Rattus norvegicus
0.0041	-	palmitoyl-CoA	mutant N464D	Rattus norvegicus
0.0041	-	L-carnitine	mutant N464D	Rattus norvegicus
0.0049	-	palmitoyl-CoA	wild-type	Rattus norvegicus
0.0049	-	L-carnitine	wild-type	Rattus norvegicus
0.0061	-	palmitoyl-CoA	mutant M593A	Rattus norvegicus
0.0061	-	L-carnitine	mutant M593A	Rattus norvegicus
0.0063	-	palmitoyl-CoA	mutant M593E	Rattus norvegicus
0.0063	-	L-carnitine	mutant M593E	Rattus norvegicus
0.0074	-	palmitoyl-CoA	mutant M593S	Rattus norvegicus
0.0074	-	L-carnitine	mutant M593S	Rattus norvegicus
0.0151	-	palmitoyl-CoA	mutant A478G	Rattus norvegicus
0.0151	-	L-carnitine	mutant A478G	Rattus norvegicus
0.0243	-	palmitoyl-CoA	mutant C608A	Rattus norvegicus
0.0243	-	L-carnitine	mutant C608A	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P32198	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoyl-CoA + L-carnitine	-	Rattus norvegicus	CoA + L-palmitoylcarnitine	-	?

Synonyms

Synonyms	Comment	Organism
carnitine palmitoyltransferase I	-	Rattus norvegicus
CPT I	-	Rattus norvegicus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0087	-	IC50 0.0087 mM for mutant enzyme N464D	Rattus norvegicus	malonyl-CoA
0.0123	-	IC50: 0.0123 for wild-type enzyme	Rattus norvegicus	malonyl-CoA
0.015	-	IC50 0.015 mM for mutant enzyme T314S	Rattus norvegicus	malonyl-CoA
0.0275	-	IC50 0.0275 mM for mutant enzyme C608A	Rattus norvegicus	malonyl-CoA
0.0395	-	IC50 0.0395 mM for mutant enzyme A478G	Rattus norvegicus	malonyl-CoA
0.155	-	IC50 0.155 mM for mutant enzyme M593A	Rattus norvegicus	malonyl-CoA
0.319	-	IC50 0.319 mM for mutant enzyme M593 mM	Rattus norvegicus	malonyl-CoA

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Release 2023.1 (January 2023)