EC Number   |
pH Optimum Minimum |
pH Optimum Maximum |
Reference |
|---|
   3.1.21.1 | -999 |
- |
DNase I is a neutral enzyme |
650035 |
| 3.1.21.1 | 5.4 |
- |
in 33 mM sodium phosphate buffer |
134228 |
| 3.1.21.1 | 5.5 |
- |
in 33 mM acetate buffer, 6 times faster than in phosphate buffer |
134228 |
| 3.1.21.1 | 5.8 |
- |
in the presence of Mg-EGTA |
134225 |
| 3.1.21.1 | 6 |
- |
in the presence of Mg2+, dependent on bivalent metal ions |
134231 |
| 3.1.21.1 | 6 |
8 |
Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum |
650419 |
| 3.1.21.1 | 6.2 |
- |
in 25 mM cacodylate hydrochlorid and 3 mM Co2+ |
134216 |
| 3.1.21.1 | 6.4 |
- |
in the presence of Co2+ in 50 mM cacodylate-HCl buffer |
134218 |
| 3.1.21.1 | 6.5 |
- |
- |
134203, 134214, 651811 |
| 3.1.21.1 | 6.5 |
- |
DNase I type-2 enzyme |
697387 |
