Inhibitors | Comment | Organism | Structure |
---|---|---|---|
G-actin | complete inhibition at 0.05 mg/ml | Cavia porcellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Cavia porcellus | |
Mn2+ | - |
Cavia porcellus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
sucrose density gradient centrifugation, gel filtration | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Cavia porcellus | - |
5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Cavia porcellus |
Purification (Comment) | Organism |
---|---|
near homogeneity by affinity chromatography | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 7.5, 5 mM CaCl2, 10% v/v glycerol, 6 months | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | degradation of native DNA is 80%, that for denatured DNA 70% | Cavia porcellus | 5'-phosphooligonucleotides + ? | 88% of products are longer than tetranecleotides | ? | |
double-stranded DNA + H2O | - |
Cavia porcellus | 5'-phosphooligonucleotides + ? | - |
? | |
heat-denatured DNA + H2O | - |
Cavia porcellus | 5'-phosphooligonucleotides + ? | - |
? | |
supercoiled plasmid DNA + H2O | - |
Cavia porcellus | linear DNA + ? | superhelical form converted to circular-relaxed and linear forms | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
in the presence of Co2+ in 50 mM cacodylate-HCl buffer | Cavia porcellus |
7.5 | - |
in the presence of Mn2+ in 50 mM Tris-HCl buffer | Cavia porcellus |