Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.21.1 extracted from

  • Shiokawa, D.; Tanuma, S.
    Characterization of human DNase I family endonucleases and activation of DNase gamma during apoptosis (2001), Biochemistry, 40, 143-152.
    View publication on PubMed
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
transient ectopic expression in HeLa S3 cells, no toxic effect on host cells, expression in human embryonic kidney 293 cells as His-tagged enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
aurintricarboxylic acid i.e ATA, a general inhibitor of nucleases, weak inhibition Homo sapiens
G-actin specific, strong inhibition Homo sapiens
Ni2+
-
 Homo sapiens
Zn2+
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, DNase I is a Ca2+/Mg2+-dependent enzyme, synergism Homo sapiens
Co2+ activates Homo sapiens
Mg2+ activates, DNase I is a Ca2+/Mg2+-dependent enzyme, synergism Homo sapiens
Mn2+ activates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32498
-
 x * 37000, recombinant secreted enzyme, SDS-PAGE, x * 32498, sequence calculation Homo sapiens
37000
-
 x * 37000, recombinant secreted enzyme, SDS-PAGE, x * 32498, sequence calculation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Homo sapiens
-
 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
additional information Homo sapiens DNase I is not involved in aopoptosis, but DNase lambda is ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P24855 different enzyme types of the human DNase I family: DNase I, DNase X, DNase lambda, and DNASIL2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains 2 potential N-glycosylation sites Homo sapiens
proteolytic modification enzyme sequence contains a precursor peptide Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from 293 cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney high expression level Homo sapiens
-
additional information tissue distribution of enzymes of the DNase I family, complete overview Homo sapiens
-
pancreas very high expression level Homo sapiens
-
small intestine high expression level Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
 Homo sapiens 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
dsDNA + H2O in presence of Mg2+ and Ca2+ under neutral conditions Homo sapiens 5'-phosphodinucleotide + 5'-phosphooligonucleotide production of 3'-OH and 5'-phosphate ends ?
additional information DNase I is not involved in aopoptosis, but DNase lambda is Homo sapiens ?
-
 ?
supercoiled DNA + H2O in presence of Mg2+ and Ca2+ under neutral conditions Homo sapiens 5'-phosphodinucleotide + 5'-phosphooligonucleotide production of 3'-OH and 5'-phosphate ends ?

Subunits

Subunits Comment Organism
? x * 37000, recombinant secreted enzyme, SDS-PAGE, x * 32498, sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
DNase I
-
 Homo sapiens
More enzyme belongs to the DNase I family Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 DNase I is a neutral enzyme Homo sapiens
6.8
-
 DNase I Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens